藿香正气软胶囊对肠屏障功能保护作用的机理研究

目的 :探讨藿香正气软胶囊对肠屏障功能的保护作用。方法 :Wistar大鼠 5 0只 ,建立肢体缺血 再灌注模型。中药组分别于造模前经口给予藿香正气软胶囊 ;肠组织超微结构观察、肠黏液分泌及肥大细胞量化分析、测定血清一氧化氮浓度。结果 :肢体缺血 再灌注后 ,肠道屏障功能明显受到损伤和破坏。藿香正气软胶囊可有效减轻肠黏膜损伤 ;可显著降低大鼠血清NO浓度。结论 :藿香正气软胶囊对肢体缺血 再灌注损伤时肠道屏障功能具有明显的保护作用。

Experimental study on protecting intestinal barrier function of Huoxiang Zhengqi soft capsule

Objective: To study the effect of Huoxiang Zhengqi soft capsule on protecting intestinal barrier. Method: Lower limb ischemic reperfusion model was induced in wistar rats. The Chinese medicine groups were administered orally with Huoxiang Zhengqi soft capsule before inoculation respectively. The small intestinal histopathology and ultrastructural of rats were observed with optical microscope and electronic microscope respectively. Mucus was observed under AB-PAS staining, mast cells were studied under toluidine blue staining,and serum NO was determined. Result: After Lower limb ischemic reperfusion, the intestinal barrier function was severely damaged. Huoxiang Zhengqi soft capsule could significantly reduce the levels of the serum NO ( P <0.01). Conclusion: Huoxiang Zhengqi soft capsule has apparent protective effect on the intestinal barrier function in lower limb ischemic reperfusion rats.