| 细菌性感染PCR诊断方法的建立及其应用研究 |
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| 引用本文： | 张宁,孟欣,惠凌云,邓凯.细菌性感染PCR诊断方法的建立及其应用研究[J].中华医院感染学杂志,2011,21(14):3079-3081. |
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| 作者姓名： | 张宁 孟欣 惠凌云 邓凯 |
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| 作者单位： | 1. 西安交通大学医学院第一附属医院检验科,陕西,西安,710061;西安交通大学医学院免疫学与病原生物学系,环境与疾病相关基因教育部重点实验室,陕西,西安,710061
2. 西安交通大学医学院第一附属医院检验科,陕西,西安,710061 |
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| 基金项目： | 西安交通大学医学院第一附属医院科研基金项目(2009YK12) |
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| 摘 要： | 目的通过通用引物PCR,为建立一种实用、快速可靠细菌感染PCR检测方法奠定基础。方法收集100例疑似血流感染患者的血液进行细菌培养,同时检测细菌16S rRNA基因,测定和分析所得DNA序列,对培养结果与PCR结果进行比较;采用倍比稀释法进行PCR灵敏度检测。结果 100份血液标本中细菌培养8份阳性,阳性率为8.0%;PCR法19份阳性,阳性率为19.0%,细菌培养与PCR检测结果比较,差异有统计学意义(χ2=9.09,P<0.05);测序结果显示DNA序列与GenBank公布的基因序列同源性很高,PCR可检测范围至1.5×102CFU/ml。结论只要严格控制污染,通过16S rRNA基因PCR技术对疑似血流感染的临床诊断具有重要意义。
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| 关 键 词： | 细菌性感染 聚合酶链反应 16S核糖体核糖核酸 |
Establishment and application of PCR methods in diagnosing bacterial infection |
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| ZHANG Ning,MENG Xin,HUI Ling-yun,DENG Kai.Establishment and application of PCR methods in diagnosing bacterial infection[J].Chinese Journal of Nosocomiology,2011,21(14):3079-3081. |
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| Authors: | ZHANG Ning MENG Xin HUI Ling-yun DENG Kai |
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| Institution: | ZHANG Ning,MENG Xin,HUI Ling-yun,DENG Kai(The First Affiliated Hospital of Medical College of Xi'an Jiaotong University,Xi'an,Shannxi 710061,China) |
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| Abstract: | OBJECTIVE To develop a rapid and reliable method for the detection of bacteria infections with universal primer PCR technology.METHODS A total of 100 patients with suspected bacterial infections were collected and performed bacteria culture and analyzed by PCR for bacterial 16S ribosomal RNA gene.The DNA fragments were directly sequenced and compared with the 16S ribosomal RNA sequences reported in GenBank.At the same time,the results of two methods were compared.The PCR sensitivity test was done by the met... |
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| Keywords: | Bacterial infections Polymerase chain reaction 16S rRNA |
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