转染Caspase—1基因的人胃癌细胞生物学特性的研究

目的 探讨转染Caspase-1基因对胃癌细胞生物学特性的影响.方法 将人凋亡基因Caspase-1的重组逆转录病毒导入胃癌细胞株X-108,对转染阳性细胞的体外增殖、细胞培养液中CEA含量及裸鼠致瘤性进行分析.结果 导入的Caspase-1基因虽不能直接诱导细胞凋亡,但G_1期细胞数目增多,S期细胞数目减少.转染细胞培养液CEA含量有下降趋势.胃癌细胞株X-108-Caspase-1体外生长速度明显受抑,裸鼠体内致瘤性降低,肿瘤生长缓慢.结论Caspase-1不能促使X-108胃癌细胞凋亡,但可以使其增殖受抑,肿瘤细胞的恶性程度降低.

BIOLOGICAL CHARACTERITICS OF THE HUMAN GASTRIC CANCER CELL LINE TRANSFECTED WITH CASPASE-1 GENE

Objective :To investigate the biological characteristics of human gastric cancer transfected with Caspase-1 gene. Methods : Recombinant retroviral vector with human Caspase-1 cDNA was transferred into human gastric cell line X-108.The proliferation tumorigenicity, cycle of the delivered cell line and the CEA concertration in the super-nate of cell culture were analyzed, respectively. Results: Apoptosis was not induced, but the cell number of G1 stage increased, the S stage decreased, the CEA concentration in supernate also decreased, the proliferation and the tumorigenicity cf the gastric cell line transfected with Caspase-1 gene were considerably inhibited. Conclusion : Though apoptosis of the gastric cancer cell line X-108 transfected with Caspase-1 gene was not induced,the proliferation was inhibited.