异汉防己碱增强阿霉素诱导乳腺癌细胞凋亡的实验研究

目的:探讨异汉防己碱增强阿霉素诱导耐阿霉素乳腺癌细胞系MCF-7/DOX凋亡的效应和机制。方法:采用MTT法检测异汉防己碱、阿霉素及两者联合对MCF-7/DOX细胞增殖的抑制作用,用流式细胞术定量分析MCF-7/DOX细胞的凋亡率,用Westem blot技术检测Bax及Bcl-2表达。结果:异汉防己碱可增强阿霉素对MCF-7/DOX细胞增殖的抑制作用,并可诱导细胞凋亡;异汉防己碱联合阿霉素可上调Bax表达,降低Bcl-2表达。结论:异汉防己碱联合阿霉素是通过上调Bax/Bcl-2比值而增强阿霉素诱导癌细胞凋亡的作用。

Study on isotetrandrine enhancing the effect of doxorubicin - induced apoptosis on breast cancer cell lines MCF-7/DOX

Objective:To explore the mechanisms and the enhanced sensitization to chemotherapeutic agent doxorubicin-induced apoptotic effect on doxorubicin-resistant human breast cancer cell lines MCF-7/DOX by isotetrandrine.Methods:The proliferative inhibitory effect was assessed by MTT assay.Cells apoptotic rate was assessed by fluorescence-activated cell sorting(FACS) analysis.The expression levels of Bax and Bcl-2 were determined by Western blot.Results:Isotetrandrine rendered MCF-7/DOX cell lines more susceptible to doxornbicin-induced proliferative inhibition,which further attributed to apoptotic induction.Furthermore,combination of isotetrandrine and doxorubicin made the expression of Box up-regulation and the expression of Bcl-2 down-regulation.Conclusion:Combination of isotetrandrine and doxorubicin rendered doxorubicin-resistant human breast cancer cells lines MCF-7/DOX more susceptible to doxorubicin-induced apoptosis by up-regulation of Box /Bcl-2.