Box-Behnken效应面法优化菊花提取工艺

目的：优选菊花的提取工艺条件。方法：选择提取时间、乙醇体积分数、加醇量为自变量，木犀草素、芹菜素、绿原酸得率的总评“归一值”为因变量，采用Box-Behnken效应面法优选菊花的醇提工艺并对该工艺进行预测分析。结果：最佳提取工艺为加12倍量80%乙醇提取110 min；木犀草素、芹菜素、绿原酸平均得率分别为1.64%，0.94%，3.95%，实测OD=0.979 6（RSD 1.83%），与预测值（0.981 6）的偏差0.204%。结论：优选的提取工艺稳定可行且可预测性强。

Optimization of Extraction Process of Chrysanthemi Flos by Box-Behnken Response Surface Methodology

Objective: To optimize extraction process of Chrysanthemi Flos. Method: Based on single factor test, taking extracting time, ethanol concentration and amount as independent variables, overall desirability(OD) of yield of luteolin, apigenin and chlorogenic acid as dependent variable, Box-Behnken response surface methodology was applied to optimize extraction conditions. Result: Optional extraction process was as follows:extracted 110 min with 12 times the amount of 80% ethanol;Average yields of luteolin, apigenin and chlorogenic acid were 1.64%, 0.94% and 3.95%, respectively, deviation between the measured value(0.979 6) and the predicted value(0.981 6) of OD was 0.204%. Conclusion: This optimized extaction technology was simple and reliable with high predictability.