| 实验感染来亨鸡及蚊虫体内西方马脑炎病毒RT-PCR检测 |
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| 引用本文： | 汪中明,赵彤言,吴渊明,董言德. 实验感染来亨鸡及蚊虫体内西方马脑炎病毒RT-PCR检测[J]. 寄生虫与医学昆虫学报, 2007, 14(4): 213-217 |
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| 作者姓名： | 汪中明 赵彤言 吴渊明 董言德 |
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| 作者单位： | 军事医学科学院微生物流行病研究所,病原微生物生物安全国家重点实验室,北京,100071;军事医学科学院微生物流行病研究所,病原微生物生物安全国家重点实验室,北京,100071;军事医学科学院微生物流行病研究所,病原微生物生物安全国家重点实验室,北京,100071;军事医学科学院微生物流行病研究所,病原微生物生物安全国家重点实验室,北京,100071 |
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| 基金项目： | 国家部科技攻关课题(2003BA712A09-02) |
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| 摘 要： | 本研究建立了西方马脑炎病毒的RT-PCR检测方法,应用于实验感染西方马脑炎病毒的来亨鸡血液及蚊虫样本中的病毒核酸检测。结果从感染病毒的来亨鸡血液及蚊虫样本中均能扩增出与预期分子量一致的特异性条带,经序列测定证明,与西方马脑炎71V-1658(NC-003908)病毒株核酸序列有较高的同源性。表明所建立的RT-PCR检测方法能够快速敏感地检测出实验感染来亨鸡血液样本及蚊虫体内西方马脑炎病毒。
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| 关 键 词： | 西方马脑炎病毒 RT-PCR 蚊虫 来亨鸡 |
| 修稿时间： | 2006-11-20 |
DETECTION OF WESTERN EQUINE ENCEPHALITIS VIRUS IN EXPERIMENTALLY INFECTED MOSQUITOES AND LEGHORN CHICKEN BY RT-PCR |
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| WANG Zhong-Ming,ZHAO Tong-Yan,WU Yuan-Ming,DONG Yan-De. DETECTION OF WESTERN EQUINE ENCEPHALITIS VIRUS IN EXPERIMENTALLY INFECTED MOSQUITOES AND LEGHORN CHICKEN BY RT-PCR[J]. Acta Parasitologica et Medica Entomologica Sinica, 2007, 14(4): 213-217 |
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| Authors: | WANG Zhong-Ming ZHAO Tong-Yan WU Yuan-Ming DONG Yan-De |
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| Abstract: | A pair of primers were synthesized,and related RT-PCR assay was established to detect the western equine encephalitis virus(WEEV)in experimentally infected mosquitoes and Leghorn chicken.The optimal condition of RT-PCR was obtained by a serial tests.The results showed that the nuclear acid sequence of the PCR product was similar with those of 71V-1658 strain(Accession no:NC003908).The lower limit of RT-PCR detection determined using WEEV RNA extracted from serial dilutions of virus in infected mouse brain tissues was approximately 10-7 dilutions.The RT-PCR assay could be reliably used as an efficient and rapid protocol of detecting WEEV in experimentally infected mosquitoes and Leghorn chicken. |
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| Keywords: | Western equine encephalitis virus RT-PCR Mosquito Leghorn chicken |
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