哈萨克族肥胖儿童β3-肾上腺素能受体基因多态性观察

目的探讨β3-肾上腺素能受体基因(β3-AR)Trp64Arg变异与新疆哈萨克族儿童肥胖的相关性。方法选取乌鲁木齐周边地区95例6~12岁哈萨克族学龄肥胖儿童及87名非肥胖儿童，用限制性片段长度多态性方法检测被调查儿童的基因型，生化方法检测血清甘油三酯(TG)、总胆固醇(TC)、高密度脂蛋白胆固醇(HDL-C)、低密度脂蛋白胆固醇(LDL-C)、载脂蛋白Ａ(ApoA)、载脂蛋白Ｂ(ApoB)水平，并测量身高、体重。结果变异型等位基因(Ｃ)在被调查对象中出现的频率为0.194，其中男0.210，女0.160。肥胖儿童中变异等位基因(Ｃ)、Trp64Arｇ变异基因型的出现频率明显高于非肥胖者(P<0.05)。单纯性肥胖儿童与非肥胖儿童比较，血清TG、TC、LDL-C、ApoB水平均明显升高(P<0.05)。β3-AR不同基因型间血脂比较，差异无统计学意义(P>0.05)。结论哈萨克族学龄儿童中存在一定的β3-AR Trp64Arg变异，可能与哈萨克族儿童肥胖有关。

Study on Beta3-adrenergic receptor gene polymorphism in Karzak obese children in xinjiang.

ObjectiveTo investigate the association between Trp64Arg mutation of theβ3-adrenergic-receptor gene and obesity in Karzak children.MethodsTotally 95 patients with obesity and 87 controls were selected from the area around Urumqi.Genotype of the β3-AR was determined by polymerase chain reaction(PCR)and restriction fragment length polymorphism methods (RFLP).Polymorphism(PCR RFLP)and serum concentration of triglyceride,total cholesterol,low density lipoprotein cholesterol (LDL-C),apolipoprotein A(ApoAI),apolipoprotein B(ApoB) and high density lipoprotein cholesterol (HDL-C) were measured with biochemical method.In the mean time,the weight and height were detected.ResultsThe mutative Callee was 0.194 for all the children,0.210 for boys and 0.160 for girls.The frequency of callee and the genotype Trp64Arg was higher in obese children.There were higher levels of the lipids in Karzak obese children than in the control group,excluding apolipoprotein A and high density lipoprotein cholesterol.There was no apparent difference between the genotype Trp64Arg and Trp64Trp in serum concentration of lipids.ConclusionThere is distribution of β3-AR Trp64Arg mutation in Karzak school-aged children in XinJiang.These results suggest that the Trp64Arg mutation of β3-adrenergic-receptor gene may be associated with Karzak children obesity.