Chromosomal Analysis of Purified B-Chronic Lymphocytic Leukemia Lymphocyte Cultures: Comparison with Whole Blood Cultures and in Situ Hybridization |
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Authors: | Andre Brizard Franchise Brizard Brigitte Dreyfus Franck Morel Jean-Claude Lecron Joseph Tanzer Jean-Louis Preud'homme |
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Abstract: | Chromosomal analysis of stimulated whole blood cells and purified B lymphocytes was performed in 13 stage A(0) and 1 stage C(1V) chronic lymphocytic leukemia (B-CLL) patients. Abnormal clones were found in 6 cases in purified B lymphocytes cultures and in a single one in whole blood cultures. In situ hybridization with a chromosome 12 probe was in accordance with the chromosomal analysis of purified B-CLL lymphocytes and not with the results obtained using whole blood culture. Cytogenetic analysis of isolated B cells is simple and sensitive. It enhances the detection of abnormal clones in B-CLL and applied to larger series of patients, it should allow a precise evaluation of the incidence of chromosomal abnormalities in CLL and of their clinical (prognostic) significance. |
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Keywords: | B-CLL Purified B lymphocytes Karyotype In situ hybridization |
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