Electron microscopy of endothelial cells in culture: I. Transmission electron microscopy

Summary Endothelial cells in culture can be identified by structural and functional characteristics. To examine the ultrastructure of the undisturbed endothelial cell monolayer, methods are described for in situ fixation and embedment in the tissue culture flask. Subsequent sectioning and staining for electron microscopy are carried out without detachment of the cell layer from the flask.