不同免疫调节剂对烫伤大鼠巨噬细胞功能的影响

目的 探讨不同免疫调节剂对烫伤大鼠腹腔巨噬细胞（Mφ）功能的影响。方法 应用McAb APAAP桥联酶标法、琼脂溶菌板法和MTT比色法等技术，分别测定了各组烫伤大鼠不同时相腹腔Mφ各种功能的变化。结果 ①烫伤后大鼠腹腔Mφ表面Ia抗原表达率降低，抗原提呈能力减弱，对念珠菌的噬菌率下降，溶菌酶活力减低，TNF分泌增高，且伤后第10天比第5天更明显。以上结果与正常组比较，差异有非常显著意义（P＜0．01）。②经不同免疫调节剂治疗后，各组烫伤大鼠腹腔Mφ各种功能明显改善，各项指标与未用药的对照组,差异有非常显著意义（P＜0．01）。结论 烫伤后早期腹腔注射特异性免疫核糖核酸（iRNA)可明显改善伤鼠的免疫功能。

Effect of different immunomodulators on macrophage function in rats with scald injury]

OBJECTIVE: To explore effect of different immunomodulators on macrophage (M phi) functions in rats with scald injury. METHODS: The M phi functions in various groups of rats with scald on the 5th and 10th day after scald were respectively determined with methods of McAb APAAP, agar bacteriolytic plate and MTT colorimetry. RESULTS: The results showed that: 1. after scald, presenting rate of Ia antigen, ability of antigen presentation, phagocytic power to candida albicans and lysozyme ability of M phi were significantly decreased, the capacity of M phi secreting TNF was markedly increased compared with those of the normal group, and the differences were significant (P < 0.01); 2. after the therapy with immunodulators, the above functions of M phi in rats with scald improved markedly. As compared with those of the control group, differences were significant (P < 0.01). CONCLUSION: The specific immune RNA, which is administered by intraperitoneal injection early after scald, can markedly improve immune functions of rats with scald.