瘢痕成纤维细胞的钙网蛋白表达研究

目的 观察钙网蛋白（ＣＲＴ）在瘢痕及下沉皮肤成纤维细胞内的表达情况。方法 采用免疫细胞化学染色和原位ＥＩＳＡ技术对体外培养的瘢痕及正常皮肤成纤维细胞进行ＣＲ贮位及表达水平研究。结果 瘢痕和正常皮肤成纤维细胞在处于增殖生长状态时，胞浆及核内均秀不同程度的ＣＲＴ表达，其中瘢痕成纤维细胞表达ＣＲＴ水平（Ａ：１．９７±０．１５，ＥＬＩＳＡ４９２ｎｍ）明显高于正常皮肤成纤维细胞水平（Ａ：１．４３±０．１２）

Study on the expression of calreticulin in hypertrophic scar-derived fibroblasts]

OBJECTIVE: To investigate the expression level and intracellular distribution of calreticulin(CRT) in hypertrophic scar-derived fibroblasts(HSF) and normal skin-derived fibroblasts(NSF). METHODS: Using anti-CRT antibody, CRT was detected in HSF and NSF by immunocytochemistry and ELISA technique. RESULTS: CRT was present in the cytoplasm and nuclei in proliferating HSF or NSF in culture, but HSF possessed significantly higher expression level of CRT than NSF(P < 0.01). When these cells (HSF and NSF) were fused, neither intracellular nor intranuclear staining for CRT was observed. CONCLUSION: In HSF and NSF, the distribution of CRT is widespread. Because of its abundant expression in HSF, we postulate that CRT as a multifunctional protein plays an important role in Ca2+ sequestering, integrin-mediated signalling and cell adhesion.