纳米化仿生诱导骨的构建及促进兔横突间融合研究

目的 探讨纳米化仿生诱导骨促进兔脊柱融合的可行性.方法 成骨化兔脂肪基质细胞(ADSCs)接种在纳米级β磷酸三钙/壳聚糖/聚已内酯基质,构建纳米化仿生诱导骨;建立兔腰5～6横突间融合模型,A组纳米化仿生诱导骨,B组自体髂骨,C组脂肪基质细胞移植,D组空白支架,E组rhBMP-2注射.行影像学、组织学、免疫组化、新骨及诱导因子定量分析等检查.结果 A组融合能力最强,融合率100%、新骨面积比(87.32±1.68)%、BMP含量及力学指标(128±19)、(331.56±3.21)N、(526.78±3.19)Nmm均高于其他组,差异有统计学意义(P＜0.05);B、C组次之,E组新骨生长不明显,融合相对延缓.D组未融合.结论 仿生诱导骨能强化骨再生,促进脊柱融合.

An experimental study on rabbit' s intertransverse process fusion promoted by mimetically osteoinductive nanoscale bone

Objective To investigate the applied value of mimetically osteoinductive bone in promoting spinal fusion of rabbits. Methods With aid of biological gels compound and dynamic (oscillating pattern) seeding, three-dimensional construction of mimetically osteoinductive bone was made by implanting adipose-derived stromal cells (ADSCs) into rhBMP-2-mediated nβ-TCP/Cs/PCL matrix. Lumbar 5-6intertranverse process fusion was established in New Zealand rabbits. The rabbits in group A were transplanted by mimetically osteoinductive bone, those in group B by bone autograft, those in groups C and E implanted by ADSCs and rhBMP-2 respectively, and those in group D given nβ-TCP/Cs/PCL matrix as blank scaffold. Histology, immunohistochemistry, new bone ratio, BMP quantification, and palpation and biomechanical examinations were performed at different periods. Results Compared with other groups,group A displayed remarkable fusion capacity with statistically significant difference according to data of new bone ratio and BMP content and biomechanical testing 100% of fusion rate, ( 87. 32 ± 1.68 ) % of new bone ratio, ( 128 ± 19) of BMP content, (331.56 ±3.21 ) N, (526. 78 ±3. 19) Nmm,P ＜0. 05].Conclusion Enhanced osteogenic potential can be achieved by using mimetically osteoinductive bone and spinal fusion can be promoted with concord pattern of osteoinductive and osteoconductive effects.