实验性大鼠肺血栓栓塞症中ICAM-1,P-选择素的变化及当归注射液的影响

目的动态观察大鼠肺血栓栓塞症(PTE)中血管内皮上细胞间黏附分子-1(ICAM-1)和P-选择素(P-selectin,Ps)的变化,探讨当归注射液对其的影响。方法颈静脉注入自体血凝块制备动物模型。将SD大鼠随机分为正常组、栓塞模型组和当归注射液治疗组。行病理分析,免疫组化和原位杂交法检测肺血管内皮上ICAM-1和Ps的蛋白表达水平和mRNA水平。结果肺栓塞后病理可见肺动脉血栓形成,炎性反应明显。肺血管内皮上ICAM-1表达及mRNA含量在栓塞后3h开始增高(P<0.01),Ps表达及mRNA含量在栓塞后1h开始增高(P<0.01)。当归注射液治疗后,病理损伤减轻,肺血管内皮上ICAM-1和Ps的水平均下降(P<0.01)。结论当归注射液可以减轻大鼠PTE时的肺损伤,这种作用可能是通过下调血管内皮上的黏附分子来实现的。

The Expression of ICAM-1, P-selectin in the Lung Tissue of Acute Pulmonary Embolism and the Effects of Angelica Injection

Objective To discuss the expression of intercellular adhesion molecule-1, P-selectin in the vascular endothelial cell and the effects of angelica injection. Methods Rats were randomly divided into control group(C-group), model group(M-group) and treatment group(T-group). Pulmonary thromboembolism(PTE) model was established by intravenous injection of auto-blood clots. The expression of ICAM-1 and P-selectin protein were evaluated by immunohistochemical method and their mRNA contents were detected by in situ hybridization. Results The notable embolism in pulmonary arteries and inflammatory cell infiltration around alveoli could be found by microscopy. The expression of ICAM-1 was elevated quickly at 3 hours after embolization(P<0.01). The expression of P-seletin was higher at 1 hour after embolization than that before embolization(P<0.01). Whereas after using angelica injection, the expression of ICAM-1 and P-selectin were significantly decreased; the pathological injury was ameliorated. Conclusion Angelica injection may alleviate pulmonary inflammation in rat pulmonary thromboembolism through inhibiting the expression of ICAM-1 and P-selectin.