| 兔眼角膜、结膜成纤维细胞的培养 |
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| 引用本文： | 张晓峰,盛伟华,杨吉成.兔眼角膜、结膜成纤维细胞的培养[J].苏州大学学报(自然科学版),2002,22(2):141-143. |
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| 作者姓名： | 张晓峰 盛伟华 杨吉成 |
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| 作者单位： | 苏州大学生物技术系 苏州215007
(张晓峰，盛伟华)，苏州大学生物技术系 苏州215007(杨吉成) |
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| 摘 要： | 目的 建立眼角膜、结膜细胞体外培养的方法。方法 采用组织块培养法培养兔眼角膜基质及结膜成纤维细胞。结果 结膜成纤维细胞在培养10天以后可以形成单层细胞。眼角膜成纤维细胞在培养瓶中培养2周后可形成单层；24孔板培养3d后细胞贴壁生长旺盛，10d形成单层。结论 眼角膜、结膜成纤维细胞组织块培养法是可行的，但在24孔培养板上眼角膜成纤维细胞比在培养瓶中贴壁快而且容易生长。
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| 关 键 词： | 角膜 结膜 成纤维细胞 细胞培养 动物实验 |
| 文章编号： | 1000-5749(2002)02-0141-03 |
| 修稿时间： | 2001年12月15 |
Rabbit Eye Corneal and Conjunctival Fibroblast Culture |
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| ZHANG Xiao-feng,SHENG Wei-hua,YANG Ji-cheng.Rabbit Eye Corneal and Conjunctival Fibroblast Culture[J].Suzhou University Journal of Medical Science,2002,22(2):141-143. |
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| Authors: | ZHANG Xiao-feng SHENG Wei-hua YANG Ji-cheng |
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| Abstract: | Object To investigate the culture technique for corneal and conjunctival fibroblast.Methods The corneal and conjunctival fibroblast for rabbits were cultured by tissue culture.Result The conjunctival fibroblast of rabbit formed a monolayer and had a fibriform arragement after 10d culture.The corneal fibroblast of rabbit formed a monolayer and had a fibriform arragement after two weeks culture in culture flasks. The cells grew very well in 24-well culture plate for 3 days culture and had a fibriform arragement after 10d culture.Conclusion Two kinds of eye cells from rabbit can be cultured successfully.It is easier to culture corneal fibroblast in 24-well plate than in flask. |
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| Keywords: | cornea conjunctive fibroblast cell culture |
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