人红细胞血型糖蛋白A单克隆抗体的初步鉴定

目的：制备高效价的人红细胞血型糖蛋白A单克隆抗体（GPA McAb)，为研制快速全血凝集试剂提供关键的双功能抗体成分。方法：首先用MNGP亚型GPA，经SPDP与小鼠血清白蛋白连接后，常规免疫Balb/c小鼠，采用杂交瘤技术制备GPA McAb；用ELISA和血凝方法对克隆进行筛选和鉴定。结果：4次融合共获3个可分泌高效价GPA McAb细胞株（3F6、5C7和5G3）。分泌的单克隆抗体均与GPA发生特异性反应，但均不引起人A、B、AB和O型红细胞凝集，属于人红细胞GPA的特异性非凝集抗体。在3株抗体中，2株为IgG1亚型，1株为IgG2亚型。结论：小分子GPA经与同种动物（免疫用动物）血清白蛋白连接后用作免疫抗原，研制的2株IgG1亚型抗体可用于全血凝集试剂中双功能抗体的制备。

Characteristics of monoclonal antibody against glycophorin A of human erythrocyte

Objectives: To study and prepare monoclonal antibody against glycophorin A of human erythrocyte(GPA McAb).This antibody is a key reagent in preparation of bispecific antibodies for rapid whole blood immunoassay. Methods: BALB/c mice received GPA antigen injection. Hybridoma was produced by traditional techniques. Hybridoma was determined with ELISA and indirect agglutination(IA) method. Results: All 3 GPA McAb reacted with GPA, and they did not autoagglutenate with four types of red cells(type A,B,O,AB).Of the 3 McAb GPA, two antibodies were IgG1,one IgG2 subtypes. Two IgG1 McAb GPA can be used in making bispecific antibody in preparation of rapid whole blood immunoassay. Conclusions: Immunogenity of GPA was enhanced after coupling with the native serum albumin of the immunized animal, and high titer GPA McAb could be easily obtained. This result is important in making bispecific antibodies in preparation of rapid whole blood immunoassay.