| 肿瘤抑制基因p53及Rb在鼻咽癌中的表达 |
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| 引用本文： | 宋毅,王炼,黄光琦,黄韧敏,袁淑兰,刘永惠.肿瘤抑制基因p53及Rb在鼻咽癌中的表达[J].四川大学学报(医学版),1997(3). |
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| 作者姓名： | 宋毅 王炼 黄光琦 黄韧敏 袁淑兰 刘永惠 |
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| 摘 要： | 为观察ｐ５３基因和Ｒｂ基因与鼻咽癌发生的关系，应用聚合酶链反应－单链构型多态分析技术（ＰＣＲ－ＳＳＣＰ技术），检测３３例鼻咽癌组织、４例鼻咽部炎性组织及３例正常鼻咽部组织中ｐ５３基因第７，８外显子的突变。结果显示，在３３例鼻咽癌中有７例存在ｐ５３基因第８外显子的突变，突变率为２１．２％。在第７外显子中未检测出突变发生。应用Ｓｏｕｔｈｅｒｎ印迹杂交，对Ｒｂ基因在鼻咽癌中存在状态进行研究。结果表明，在１３例鼻咽癌组织中有１１例鼻咽癌组织有Ｒｂ基因的缺失或明显减弱，提示ｐ５３基因突变及Ｒｂ基因的缺失而导致的基因失活与鼻咽癌的发生有密切关系。
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| 关 键 词： | p53基因 Rb基因 鼻咽癌 基因突变 基因缺失 |
The Expression of Tumor Supperssor Gene p53 and Rb Gene in Nasopharyngeal Carcinoma |
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| Song Yi,Wang Lian,Huang Guangqi,Huang Renmin,YuanShulan,Liu Yonghui.The Expression of Tumor Supperssor Gene p53 and Rb Gene in Nasopharyngeal Carcinoma[J].Journal of West China University of Medical Sciences,1997(3). |
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| Authors: | Song Yi Wang Lian Huang Guangqi Huang Renmin YuanShulan Liu Yonghui |
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| Institution: | Chengdu 610041 |
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| Abstract: | In order to observe the relation of tumor supperssor gene p53 and Rb gene to nasopharyngeal carcinoma, we investigated p53 gene mutations in exon 7 8 in 33 cases of nasopharygeal carcinoma (NPC), using single strand conformation ploymorphism analysis of PCR (PCR SSCP). The data showed p53 mutations in 7 of 33 specimens at exon 8(21.2%). No mutation in exon 7 was detected. Analysis of Rb gene in NPC tissues by southern blot revealed the deletion and loss of activity of Rb gene in 11 of 13 cases (86.6%). Our results suggest that Rb gene and p53 gene are closely associated with the tumorigenesis of NPC. |
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| Keywords: | p53 gene Rb gene Nasopharyngeal carcinome Gene mutation Gene deletion |
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