先兆子痫患者母、脐血NK细胞增殖能力及杀伤功能测定

目的:测定先兆子痫患者母、脐血NK细胞增殖能力及杀伤功能,探讨先兆子痫的病因。方法:采用MTT法和51Cr释放法分别对先兆子痫患者(n=18)和正常晚孕妇女(n=18)的母血、脐血NK细胞进行细胞增殖能力和杀伤功能测定,同时测量NK细胞含量,并以NK-92细胞作为阳性对照。结果:先兆子痫患者和正常晚孕妇女脐血NK细胞数均高于母血(P<0.05);先兆子痫患者NK细胞增殖能力高于正常晚孕组(P<0.05);2组中脐血NK细胞增殖能力高于母血(P<0.05);先兆子痫患者NK细胞杀伤能力高于正常晚孕组(P<0.05)。结论:先兆子痫患者体内NK细胞数量及功能均表现增强状态,且脐血明显高于母血,从而在妊娠过程中影响母胎免疫耐受。

Detection of proliferative and killing ability of NK cells in preeclampsia patients

Aim: The activity of NK cells in preeclampsia was studied to investigate the pathogenesis of preeclampsia. Methods: Using MTT and 51 Cr releasing technique, the proliferative and killing ability of NK cells in maternal and umbilical blood from preeclampsia patients (n=18) and normal late pregnant women (n=18) were detected. The NK-92 cell line was as the positive control. Results: The NK cell counts of umbilical blood in preeclampsia patients and normal late pregnant women were significantly higher than those of maternal blood, respectively(P<0.05); compared with that in normal late pregnant women, the proliferative ability of NK cells in preeclampsia patients was apparently increased (P<0.05). Compared with that in maternal blood, the proliferative ability of NK cells in umbilical blood from both preeclampsia patients and normal late pregnant women was dramatically increased(P<0.05). The killing ability of NK cells in preeclampsia patients was significantly higher than normal late pregnant women (P<0.05). Conclusion: Both quantity and function of NK cells in preeclampsia women are improved, and that in umbilical blood is higher than that in maternal blood. It is supposed that NK cells may affect the maternal and fetal immune tolerance during pregnancy.