| 川芎含药血清对肝星状细胞Toll样受体4及下游信号因子 MyD88表达的影响 |
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| 引用本文： | 王海兰,何娟,曹文富,陈文龙. 川芎含药血清对肝星状细胞Toll样受体4及下游信号因子 MyD88表达的影响[J]. 中国中药杂志, 2015, 40(11): 2191-2194 |
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| 作者姓名： | 王海兰 何娟 曹文富 陈文龙 |
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| 作者单位： | 重庆医科大学 附属第一医院 中西医结合科, 重庆 400016,重庆医科大学 中医药学院, 重庆 400016,重庆医科大学 附属第一医院 中西医结合科, 重庆 400016,重庆市江北区中医院, 重庆 400020 |
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| 基金项目： | 国家自然科学基金预研项目(NSFYY201108) |
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| 摘 要： | 观察川芎含药血清对脂多糖(lipopolysaccharide,LPS)刺激的大鼠肝星状细胞(hepatic stellate cell,HSC) Toll样受体4(Toll-like receptor 4,TLR4)及下游信号因子髓样分化因子88(myeloid differentiation factor 88,MyD88)表达的影响。清洁级SD大鼠给予川芎水煎剂、秋水仙碱、生理盐水连续灌胃7 d制备川芎含药血清。体外培养的肝星状细胞,除空白组外,其余各组均予LPS 1 mg·L-1刺激 24 h,各组含药血清干预后于 37 ℃,5%CO2培养箱中培养。24 h 后收集细胞,分别采用半定量 RT-PCR和Western blot法测定TLR4,MyD88的表达情况。用LPS刺激大鼠HSC后,TLR4与MyD88的mRNA和蛋白的表达均明显增加(与空白对照组比较P<0.01)。川芎含药血清干预后,TLR4,MyD88 mRNA和蛋白的表达明显减少(与模型组比较P<0.01或P<0.05),其中川芎含药血清中、高剂量组减少最为明显。川芎含药血清可通过抑制LPS诱导的HSCs中TLR4及下游信号因子MyD88的表达,参与TLR4信号通路而发挥其抗纤维化作用。
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| 关 键 词： | 川芎 肝星状细胞 脂多糖 Toll样受体4 肝纤维化 |
| 收稿时间： | 2014-11-10 |
Effect of Ligusticum wallichii-containing serum on expressions of Toll-like receptor 4 and myeloid differentiation factor 88 in hepatic stellate cells |
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| WANG Hai-lan,HE Juan,CAO Wen-fu and CHEN Wen-long. Effect of Ligusticum wallichii-containing serum on expressions of Toll-like receptor 4 and myeloid differentiation factor 88 in hepatic stellate cells[J]. China Journal of Chinese Materia Medica, 2015, 40(11): 2191-2194 |
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| Authors: | WANG Hai-lan HE Juan CAO Wen-fu CHEN Wen-long |
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| Affiliation: | Department of Integration of Traditional Chinese Medicine and Western Medicine, the First Affiliated Hospital of Chongqing Medical University, Chongqing 400016, China,College of Pharmacy, Chongqing Medical University, Chongqing 400016, China,Department of Integration of Traditional Chinese Medicine and Western Medicine, the First Affiliated Hospital of Chongqing Medical University, Chongqing 400016, China and Chongqing Jiangbei District Hospital of Traditional Chinese Medicine, Chongqing 400020, China |
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| Abstract: | |
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| Keywords: | Ligusticum wallichii hepatic stellate cell lipopolysaccharide Toll-like receptor 4 hepatic fibrosis |
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