新加良附方联合5-Fu对荷人胃癌裸鼠血清VEGF、bFGF水平的影响

目的:观察新加良附方联合5-Fu对荷人胃癌移植瘤裸鼠血清血管内皮生长因子(Vascular Endothelial Growth Factor,VEGF)、碱性成纤维生长因子(Basic Fibroblast Growth Factor,b FGF)水平的影响。方法:建立移植性人胃癌细胞(SGC-7901)裸鼠移植瘤模型,并将模型动物随机分为空白组、5-Fu组及新加良附方高、中、低剂量组、联合给药组(新加良附中剂量+5-Fu组)6组。新加良附方高、中、低剂量组给药剂量分别为10 g·d-1·kg-1、5 g·d-1·kg-1、2.5 g·d-1·kg-1;5-Fu组给药剂量为17mg·d-1·kg-1;空白组给予等量无菌生理盐水,新加良附方联合5-Fu组剂量为新加良附方中剂量(5 g·d-1·kg-1)联合应用5-Fu(17 mg·d-1·kg-1)。给药结束后,采用免疫学方法(ELISA)检测裸鼠血清中VEGF、b FGF的含量。结果:VEGF含量显示:联合给药组,5-Fu组,新加良附大、中、小剂量组裸鼠血清中含量较空白对照组均明显降低(P0.01),新加良附各剂量组对VEGF含量减低作用具有一定的剂量依赖性,联合用药组VEGF含量低于5-Fu组、新加良附中剂量组,但无统计学意义(P0.05)。b FGF含量显示:联合给药组,5-Fu组,新加良附大、中、小剂量组裸鼠血清中含量较空白对照组均明显降低(P0.01)。结论:新加良附方可以下调人胃癌裸鼠的血清中VEGF、b FGF表达水平,提示新加良附方具有一定抗胃癌新生血管生成作用。

The Effects of Modified Liang-Fu Formula Combined with 5-Fu on VEGF,bFGF Expression of Human Gastric Cancer Xenografts in Nude Mice

Objective： To observe the effect of Modified Liang-fu Formula（ MLFF） combined with 5-Fu on VEGF and b FGF expression in the serum of human gastric cancer xenografted nude mice. Methods： The animal model of SGC-7901 xenografted nude mice was established,and all the mice were randomly divided into 6 groups; namely model control group（ the same amount of normal sterile saline）,5-Fu group（ 17 mg·d^-1·kg^-1）,MLFF high-dose group（ 10 g·d^-1·kg^-1）,MLFF medium-dose group（ 5 g·d^-1·kg^-1）,MLFF low-dose group（ 2. 5 g·d^-1·kg^-1） and drug combination group（ 5 g·d^-1·kg^-1 MLFF combined with 17 mg·d^-1·kg^-1 5-Fu）.After 10 days of continuous treatment,eyeball blood was withdrew,the mice were killed,and the expression of VEGF and b FGF in the serum were detected using ELISA method. Results： Compared to model control group,the expression levels of VEGF in nude mice serum were significantly reduced in 5-Fu group,MLFF high-dose group,MLFF medium-dose group,MLFF low-dose group and drug combination group,respectively（ P〈0. 01）,while MLFF reduced VEGF expression in a dose-dependent manner. The combination group showed a lower level of VEGF compared to 5-Fu group and MLFF medium-dose group,but there was no significant difference（ P〈0. 05）. Meanwhile,compared to model control group,the expression level of b FGF in nude mice serum was significantly decreased in 5-Fu group,MLFF high-dose group,MLFF medium-dose group,MLFF low-dose group and drug combination group,respectively（ P〈0. 01）. Conclusion： MLFF can down-regulate the expression of VEGF,b FGF in the serum of human gastric cancer xenografted nude mice. It indicates that MLFF has the potential to serve as anti-angiogenesis agent for gastric cancer.