蒙医温针对局灶性脑缺血早期脑组织VEGF含量和脑水肿的影响

目的:探讨蒙医温针对大鼠局灶性脑缺血再灌注后脑组织VEGF水平、脑梗死体积,脑含水量的影响。方法:将雄性SD大鼠随机分为蒙医温针组、针刺组、尼莫地平组、假手术组、模型组5组。采用改良线栓法建立大鼠中动脉阻塞再灌注模型,各组在造模成功1 h时进行治疗,1次/d,假手术组和模型组不给于治疗。各组大鼠在术后24 h进行神经功能评分及测脑组织VEGF水平和脑梗死体积及脑含水量。结果:假手术组无神经功能缺损症状,蒙医温针组与各组比较脑组织VEGF含量有统计学意义,各组脑梗死体积、脑含水量与假手术组比较均有统计学意义,蒙医温针组脑梗死体积、脑含水量与模型组比较有统计学意义。结论:蒙医温针有改善大鼠局灶性脑梗死后的神经功能和减轻脑水肿、降低脑梗死体积作用。蒙医温针治疗缺血性脑卒中进行早期干预具有十分重要的临床意义。

Effects on Mongolian Warm Acupuncture on VEGF Levels and Cerebral Edema of Brain Tissue in Early Focal Cerebral Ischemia

Objective： To explore the effect of the Mongolian warm acupuncture on VEGF levels in brain tissue,infarct volume,brain water content in rats with cerebral ischemia after reperfusion. Methods： Male SD rats were randomly divided into Mongolian warm acupuncture group,acupuncture group,nimodipine group,sham operation group,and model group. Modified suture method was used to set up artery occlusion in rats occlusion and reperfusion model. Rats in each group except sham group and model group were treated for one hour once a day after being successfully modelled. The nerve function,VEGF levels in brain tissue,infarct volume and brain water content after 24 hours of surgery were measured. Results： The sham operation group had no nerve functional defect symptoms,and Mongolian warm acupuncture group showed significantly different outcomes in VEGF levels in the brain tissue compared with other groups; infarct volume and brain water content in each group were significantly different from those of the sham operation group,and Mongolian warm acupuncture group had significant differences in terms of infarct volume and brain water content compared with the model group.Conclusion： Mongolian warm acupuncture can improve rat neurological function after focal cerebral infarction and ease cerebral edema and reduce infarct volume effect. Mongolian warm acupuncture has very important clinical significance to treat ischemic stroke early intervention.