| 改良-差示酚硫法的建立及对淫羊藿多糖的含量测定 |
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| 引用本文： | 王超,张曜武.改良-差示酚硫法的建立及对淫羊藿多糖的含量测定[J].西北药学杂志,2011,26(4):257-259. |
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| 作者姓名： | 王超 张曜武 |
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| 作者单位： | 青岛科技大学化工学院,山东,青岛,266042 |
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| 摘 要： | 目的建立改良-差示酚硫法并测定淫羊藿多糖含量。方法在以往苯酚硫酸法、差示苯酚硫酸法的基础上进一步研究改进,建立新的可用于多糖含量测定的方法。结果改良-差示酚硫法的测定条件:样品可直接用未精制多糖提取物,反应应在2.5h内完成,测定波长λmax=490 nm,线性范围为30~400μg·mL-1,相关系数r=0.999 0,RSD=2.2%,回收率为98.9%。结论改良-差示酚硫法可用于多糖的含量测定,多糖不必进行繁琐的分离纯化,该法操作简捷、快速,精密度高。
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| 关 键 词： | 多糖 苯酚硫酸法 含量测定 淫羊藿 |
Establishment of improved-diferential phenol sulfuric method and determination of polysaccharides in Epimedium |
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| WANG Chao,ZHANG Yaowu.Establishment of improved-diferential phenol sulfuric method and determination of polysaccharides in Epimedium[J].Northwest Pharmaceutical Journal,2011,26(4):257-259. |
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| Authors: | WANG Chao ZHANG Yaowu |
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| Institution: | WANG Chao,ZHANG Yaowu(College of Chemical Engineering,Qingdao University of Science and Technology,Shandong Qingdao 266042,China) |
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| Abstract: | Objective To establish an improved-diferential phenol sulfuric method and to determine the contents of polysaccharides in Epimedium.Method On the basis of the phenol sulfuric method,the improvement was made.Results The improved-diferential phenol sulfuric method was used directly to determine polysaccharides without other purification.The operation was completed within 2.5 h.The calibration curve was linear in the range from 30 μg·mL-1 to 400 μg·mL-1 with r=0.999 0 and the average recovery was 98.9% with RSD 2.2%.Conclusion The improved-diferential phenol sulfuric method is rapid,and can be used to determine polysaccharides directly |
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| Keywords: | polysaccharides phenol sulfuric method content determination Epimedium |
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