胶束毛细管电泳法测定天山花楸果实中苦杏仁苷的含量

目的建立了一种简单、快速分析天山花楸果实中苦杏仁苷含量测定的胶束毛细管电泳法(MEKC)。方法采用熔融石英毛细管(58.5 cm×75μm,有效长度为48.5 cm),缓冲体系为70 mmol.L-1的硼砂-200 mmol.L-1的十二烷基硫酸钠(pH 9.50);分离电压10 kV(+)→(-);电泳时间40 min;电泳温度25℃;压力进样0.5 psi×10 s;柱上检测UV 215 nm(二极管阵列检测器)。结果苦杏仁苷在20 min内得到较好分离,在25~225μg.mL-1范围内与峰面积线性关系良好,线性方程为:Y=776.66X-6 637.9(r=0.999 7),样品平均回收率为100.8%,RSD值为2.8%。结论该方法简单、快速,结果准确可靠,可用于天山花楸中氰苷类成分测定及质量控制。

Determination of the content of amygdalin in Sorbus tianschanica Rupr.fruit by micellar electrokinetic chromatography

Objective To establish a simple and rapid micellar electrokinetic chromatography method （MEKC） which can determine the content of amygdalin in Sorbus tianschanica Rupr. fruit. Methods Bare fused-silica capillary （58. 5 cm× 75 μm, effective length of 48.5 cm） was used;the buffer was composed of 70 mmol ·L^-1 sodium borate-200 mmol · L^-1 SDS （prig. 50） ;running voltage at 10 kV;polarity from anode （inlet） to cathode （outlet） ;separation time 40 min;temperature 25 ℃ ;sampling 0.5 psi×10 s;on line detection at 215 nm （PDA）. Results The linear range of amygdalin was from 25-225μg· mL^-1 ,with linear regression e- quation was Y = 776.66 X - 6 637.9 （r = 0. 999 7 ）, and the average recovery was 100.8%, with RSD 2.8%. Conclusion The method is simple,rapid,accurate and reliable,and can be applied for the quality control of cyanogenic glycosides.