抗-Dib血型不合致新生儿溶血病一例

分析1例怀疑由抗-Di^b抗体引起的新生儿溶血病(HDFN)。患儿出生两天,诊断为贫血,高胆红素血症。患儿父母均为O型,RhD阳性,排除ABO、RhD溶血。患儿母亲抗体鉴定实验结果怀疑为抗-Di^b抗体引起的HDFN,由于市面上缺乏检测Dib抗原的试剂,导致抗-Di^b抗体引起的HDFN不易被发现,使用序列特异引物引导的PCR反应及聚合酶链反应-直接测序法检测患儿及父母Di^a、Di^b抗原。结果显示该患儿血型为Di(a+b+),患儿母亲血型为Di(a+b-),患儿父亲血型为Di(a-b+),该患儿所患HDFN为罕见抗体抗-Di^b抗体所致。

A case of hemolytic disease of the fetus and newborn related to anti-Dib

A case of hemolytic disease of the fetus and newborn(HDFN)caused by anti-Di^b was analyzed in this study.The infant patient was diagnosed with anemia and hyperbilirubinemia two days after birth.Laboratory tests performed on the parents were positive for type O and RHD,so the possibility of ABO and RHD hemolysis were ruled out.The antibody screening and identification test results of the mother showed that the hemolytic disease of the newborn was caused by anti-Dib.Due to the lack of the Di^b antigen detecting reagents,it is difficult to detect HDFN caused by anti-Di^b.Genotyping on the family for Di^a and Di^b was performed by using the polymerase chain reaction with sequence-specific primer and the polymerase chain reaction-sequence-based typing methods.The sequencing results showed a Di(a+b-)genotype for the mother,Di(a-b+)for the father and Di(a+b+)genotypes for the baby.This case of HDFN was caused by the rare antibody anti-Di^b.