rhG-CSF动员对供者血浆S1P浓度的影响

1-磷酸鞘氨醇（SIP）是具有广泛生物学效应的磷脂代谢产物，多种生长因子均能通过影响细胞鞘氨醇激酶（SphK）活性而调节SIP的生成，重组人粒细胞集落刺激因子（dIG—CSF）是一种广泛用于外周血造血干细胞动员的一种细胞因子。本研究主要了解rhG—CSF动员对供者血浆SIP含量的影响。分离供者rhG—CSF动员前和动员第5天外周血单个核细胞和血浆，用RT—PCR检测动员前后单个核细胞SphK mRNA表达情况，并应用γ-^32P-ATP掺入法和SphK酶促反应检测动员前后血浆SIP变化。结果表明，供者动员前后外周血单个核细胞均表达SphK mRNA；在rhG—CSF动员前后供者血浆中均含有SIP，且SIP浓度在rhG—CSF动员前和动员后第5天无明显变化（P〉0．05）。结论：rhG—CSF动员对供者血浆SIP含量无明显影响。

Impact of rhG-CSF on Sphingosine 1-phosphase Concentration in Blood Plasma of Donors

Sphingosine 1-phosphate (S1P), which can be impacted by different growth factors through sphingosine kinase (SphK), is a bioactive lipid produced by metabolism of sphingolipid with various biological responses. Recombinant human granulocyte-colony-stimulating factor(rhG-CSF) have been used widely in peripheral blood stem/progenitor cell mobilization. This study was aimed to investigate the effects of rhG-CSF on S1P concentration in plasma of donors. The peripheral blood mononuclear cells and blood plasma were collected from 8 peripheral blood progenitor cell donors before mobilization and on the fifth day of mobilization with rhG-CSF. The SphK mRNA expression of blood mononuclear cells were detected by RT-PCR. The changes of S1P concentration were assayed with SphK enzyme catalyzed reaction. The results showed that both kinds blood mononuclear cells collected before and after rhG-CSF mobilization expressed SphK mRNA. The S1P concentration is low in donor's plasma both before and after mobilization with rhG-CSF, and there was no markedly change of S1P concentration in plasma before and after mobilization(P>0.05). In conclusion, mobilization with rhG-CSF does not impact S1P concentration in donors' plasma.