胆红素脑病仔鼠血液中NSE、S-100及MBP含量变化的动态研究

目的:探讨胆红素脑病新生大鼠血液中神经元特异性烯醇化酶(NSE)、S-100蛋白(S-100)及髓鞘碱性蛋白(MBP)的含量变化,筛选胆红素脑病早期诊断的客观指标。方法:采用7日龄Wister大鼠腹腔注射胆红素200mg/kg制备胆红素脑病动物模型。采用放射免疫法、酶联免疫法动态观察胆红素脑病新生大鼠血液中NSE、S-100及MBP含量的变化。结果:血液中NSE、S-100及MBP水平都有升高,但各项指标升高的时间和幅度并不一致。与对照组比较,实验组血液中MBP含量变化出现较早,在造模后6h即有一定程度的增加(P<0.05);NSE、S-100在12—24h都有明显增加(P<0.05)。结论:血液中NSE、S-100可反映胆红素脑病仔鼠神经元和神经胶质的损伤程度,是判定胆红素脑病的可靠指标;血液中MBP升高最早,是早期诊断胆红素脑病神经损伤的敏感生化指标。

The dynamic changes of blood NSE,S-100 and MBP in newborn rats with bilirubin encephalopathy

Objective:To analyse the dynamic changes of neuron specific enolase(NSE), S-100 protein(S-100) and myelin basic protein(MBP) levels in blood in newborn rats with bilirubin encephalopathy and evaluate their significance for early diagnosis.Method:The model was established by administered bilirubin intraperitoneally (200mg/kg) in newborn rats. The blood samples were taken from 8 rats at the sequential time points of 6h, 12h and 24h after the model was established. Eight normal rats were used as control. Values of S-100, NSE and MBP in blood were measured by RIA and ELISA.Result:All three biochemical markers significantly increased in newborn rats with bilirubin encephalopathy compared with controls. The values of NSE and S-100 increased during 12-24h after bilirubin encephalopathy. The values of MBP had substantial change at 6h. Conclusion:NSE and S-100 may reflect the degree of injury of neuron and neurogliocyte and are reliable indexes to determine bilirubin encephalopathy. MBP increasing earliest among the three indexes is a sensitive marker to diagnose bilirubin encephalopathy early.