益气活血软坚解毒含药血清诱导人肝癌细胞系Bel-7402细胞的凋亡

目的:研究益气活血软坚解毒(YHRJ)含药血清对人肝癌细胞系Bel-7402生长抑制及诱导凋亡作用.方法:将YHRJ含药血清作用于人肝癌细胞系Bel-7402细胞,应用MTT检测对肝癌细胞生长抑制作用,倒置显微镜、荧光显微镜、激光共聚焦扫描显微镜等影像学方法观察细胞形态学变化,以及PI染色单染、AnnexinV-PI双染后,流式细胞术检测对细胞周期影响及诱导细胞凋亡程度.结果:MTT法检测结果显示:YHRJ含药血清具有抑制Bel-7402肿瘤细胞生长作用(其中20%YHRJ等效剂量抑制率49.1%,与NS比,P<0.01);荧光显微镜及激光共聚焦显微镜可观察到典型的凋亡形态学变化.流式细胞术检测结果,细胞周期出现G0/G1期阻滞,并出现典型的凋亡峰,AnnexinV-PI双染法检测到早期及中晚期细胞凋亡.结论:YHRJ含药血清有抑制人肝癌细胞系Bel-7402细胞生长并有诱导细胞凋亡作用.

Growth inhibitory and apoptosis inducing effect on hepatocellular carcinoma Bel-7402 cell line by serum from rabbit fed with Yiqi Huoxue Ruanjian Jiedu decoction

AIM: To investigate the growth inhibitory and apoptosis inducing effects on hepatocellular carcinoma Bel-7402 cell line by serum from rabbit fed with Yiqi Huoxue Ruanjian Jiedu decoction(YHRJ decoction).METHODS: Serum was collected from New Zealand rabbits fed with YHRJ decoction, and used for Bel-7402 cell culture. Cell growth inhibition was assayed by MTT method. Invert, fluorescence, and laser microscopes were applied to examine cell morphology. Cell cycle and apoptosis were detected by annexinV and prop-idium iodide (PI) double staining and ? ow cytometry.RESULTS: Serum from YHRJ-fed rabbits suppressed Bel-7402 cell growth. The inhibitory rate was 49.1%, when 20% serum was added to the culture. Typical apoptosis of morphological changes were also ob-served in YHRJ serum treated cells. The G0/G1 arrest and apoptosis peak were shown by flow cytomety.Early, medium/late-term apoptosis was detected by annexinV-PI double staining.CONCLUSION: YHRJ decoction containing serum can inhibit growth and induce apoptosis of Bel-7402 cells.