| 绿色荧光蛋白小鼠骨髓间充质干细胞的体外培养及成骨诱导 |
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| 引用本文： | 董世武,应大君,糜建红,王廷华,羊惠君. 绿色荧光蛋白小鼠骨髓间充质干细胞的体外培养及成骨诱导[J]. 解剖学报, 2004, 35(5): 555-557 |
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| 作者姓名： | 董世武 应大君 糜建红 王廷华 羊惠君 |
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| 作者单位： | 第三军医大学解剖学教研室,国家教育部生物力学与组织工程重点实验室生物力学研究室,重庆,400038;昆明医学院神经科学研究所,昆明,650031;四川大学华西医学中心解剖学教研室,成都,610041 |
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| 基金项目： | 国家自然科学基金资助项目 |
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| 摘 要： | 目的分离培养绿色荧光蛋白(GFP)小鼠骨髓间充质干细胞(MSCs)并成骨诱导分化。方法密度梯度离心法分离GFP小鼠MSCs，体外传代扩增，条件培养基成骨方向诱导，利用荧光显微镜、HE染色、细胞化学和免疫组织化学方法进行观察。结果MSCs在传代和诱导分化过程中稳定表达GFP，经成骨方向诱导10d后，出现大量碱性磷酸酶和骨钙素染色阳性细胞。结论成功分离了GFP小鼠MSCs，并体外向成骨方向诱导分化。
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| 关 键 词： | 间充质干细胞 体外培养 绿色荧光蛋白 小鼠 |
CULTIVATION AND OSTEOBLASTIC DIFFERENTIATION OF MESENCHYMAL STEM CELLS ON GFP-EXPRESSING MOUSE IN VITRO |
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| DONG Shi-wu+,YING Da-jun ,MI Jian-hong+,WANG Ting-hua+,YANG Hui-jun+. CULTIVATION AND OSTEOBLASTIC DIFFERENTIATION OF MESENCHYMAL STEM CELLS ON GFP-EXPRESSING MOUSE IN VITRO[J]. Acta Anatomica Sinica, 2004, 35(5): 555-557 |
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| Authors: | DONG Shi-wu+ YING Da-jun MI Jian-hong+ WANG Ting-hua+ YANG Hui-jun+ |
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| Affiliation: | DONG Shi-wu+1,YING Da-jun 1*,MI Jian-hong+1,WANG Ting-hua+2,YANG Hui-jun+3 |
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| Abstract: | Objective To isolate the bone marrow mesenchymal stem cells(MSCs) from the GFP-expressing mouse, and to study the osteoblastic differentation of the cells. Methods MSCs were isolated by density gradient centrifugation, then the clutrued cells were induced to osteoblastic differentiation using the conditional medium. We detectd the expression of GFP and MSCs differentiation into osteoblasts by histochemistry and immunochemistry. Results The MSCs maintained the expression of GFP during expanded and induced process. After induced for 10 days, lots of alkaline phosphatase and osteoclcin staining positive cells were observed.Conclusion The MSCs of GFP-expressing mouse were successfully isolated and differentiated into ostoblasts. It may be valuable for tracing the seeding cells in tissue engineering bone. |
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| Keywords: | Mesenchymal stem cells(MSCs) Culture in vitro Green fluorescent protein(GFP) Mouse |
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