还原型谷胱甘肽保护性治疗帕金森病的机制研究

目的观察还原型谷胱甘肽(GSH)对帕金森病(PD)大鼠模型的异常行为、黑质抗氧化系统、线粒体呼吸链功能及细胞形态学的影响。方法应用6-羟基多巴胺立体定向注射制作PD大鼠模型。将24只大鼠随机分为3组(每组8只):模型组、GSH组、假手术组,分别给予相应处理,共45d,给药前后均进行行为学测试,给药后测定各组大鼠黑质区谷胱甘肽过氧化物酶(GSH-Px)、丙二醛(MDA)、活性氧(ROS)及线粒体呼吸链酶复合体水平,并行免疫组化检测。结果(1)GSH对大鼠旋转行为无明显影响(P>0.05)。(2)GSH能减轻黑质区氧化应激损伤。(3)GSH能增强黑质呼吸链酶复合体I活性。(4)免疫组化发现GSH组TH-IR神经元数量较模型组明显增多。结论GSH能减轻PD模型大鼠黑质区氧化应激损伤,并对线粒体呼吸链及细胞形态具有一定保护作用。

The experimental study of glutathione on cyto-protection function in Parkinson disease rat

Objective To observe the effects of glutathione on the abnormal behavior,nigral antioxidation system,mitochondrial respiratory-chain function and neuron morphology in model rat of PD induced by 6-hydroxydopamine (6-OHDA). Methods The rats of model PD were established through stereotoxic microinjection of 6-OHDA. The 24 rats of model after behavior test were randomly divided into 3 groups:Model group(MG),Glutathione group (GG) and sham-operated group (SG). Every group was given relevant treatment for 45 days. All rats behavior were tested before and after giving medicine . The activity of GSH-Px,content of MDA,ROS and Mitochondrionase complex I of nigra were assayed after giving medicine.The nigra form was observed by immunohistochemistry. Results (1)GSH had no effect on its rotation behavior. (2)Glutathione could decrease the nigral oxidative stress of PD rats model. (3)Glutathione could exert some protective effect on mitocondrial respiratory-chain. (4)The TH-IR neurons of GG increased in injected side compared with MG. Conclusion GSH could decrease the nigral oxidative stress of PD model rats,and exert some protective effect on mitocondrial respirtory-chain and neuron morphology.