依托泊苷对卵巢癌细胞3AO肿瘤转移抑制基因KAI1表达的影响

目的 研究依托泊苷（VP16）对人卵巢上皮性癌细胞3AO的生长抑制作用及其对细胞中肿瘤转移抑制基因KAI1表达的影响.方法 倒置显微镜下观察3AO细胞在不同浓度的药物作用下形态学变化;采用四甲基偶氮唑蓝（MTT）法观察药物对3AO细胞的生长抑制作用;运用RT-PCR、免疫细胞化学的方法 分别从转录和蛋白翻译水平检测不同药物浓度作用的3AO细胞中肿瘤转移抑制基因KAI1的表达情况.结果 药物作用下,3AO细胞体积缩小,核固缩、深染及凋亡小体出现;MTT法结果 显示药物对3AO细胞的生长抑制率呈明显的剂量依赖性;免疫细胞化学染色显示VP16能显著上调肿瘤转移抑制基因KAI1的表达;RT-PCR检测结果 显示VP16能同样增强KAI1mRNA的表达.结论 VP16不仅能抑制卵巢上皮性癌细胞的生长,而且能增强癌细胞中肿瘤转移抑制基因KAI1的表达,可能为临床上抑制肿瘤转移的药物治疗带来希望.

Effect of VP16 on the expression of metastasis suppressor gene KAI1 in human ovarian cancer cell line 3AO

Objective To investigate the growth suppressive effect of VP16 on human ovarian cancer cell line 3AO. The effect on tumor metastasis suppressor gene KAI1 expression was also detected in the cell lines. Methods The condition of 3AO cells was visualized morphologically by inverted microscopy. The inhibiting effect of drugs on cell line was tested by MTT assay and variation of KAI1 gene expression investigated by RT - PCR and immunocytochemistry （ICH） . Results The ovarian cancer cell treated by all concentration of drugs become round, in which toxic granulars and apoptosic bodies appeard. MTT assay demonstrated that high -dose VP16 could inhibit the growth of 3AO cells, which showed dose - dependent tendency. The KAI1/CD82 protein expression in different 3AO cells treated with different concentrations of VP16 was significantly up - regulated compared with the control. Result also showed the same significant up - regulations of KAI1 mRNA by RT - PCR. Conclusion Not only can VP16 inhibit the growth of human ovarian cancer cell line, but also can effectively induce KAI1 expression in human ovarian cancer cell lines, which provide us an expect of chemotherapy on tumor metastasis.