| NO引起Fyn酪氨酸激酶巯基亚硝基化的体外研究 |
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| 引用本文： | 郝凌云,齐素华,张光毅. NO引起Fyn酪氨酸激酶巯基亚硝基化的体外研究[J]. 徐州医学院学报, 2011, 31(11): 718-721 |
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| 作者姓名： | 郝凌云 齐素华 张光毅 |
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| 作者单位： | 徐州医学院生物化学与分子生物学研究中心,江苏省脑病生物信息重点实验室,徐州医学院江苏省麻醉学重点实验室,江苏,徐州,221002 |
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| 基金项目： | 国家自然科学基金重点项目，徐州医学院院课题 |
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| 摘 要： | 目的 探讨离体情况下一氧化氮（NO）对酪氨酸蛋白激酶Fyn蛋白巯基亚硝基化的影响.方法 HEK293细胞转染pcDNA3.1-Fyn质粒后,用S-亚硝基谷胱甘肽（GSNO）刺激,或共转染神经型一氧化氮合酶（nNOS）-Fyn质粒,再用A23187和7-硝基吲唑（7-NI）刺激,生物素转换法（biotin-switch method）和Western blotting检测Fyn巯基亚硝基化情况.结果 GSNO以及 nNOS在A23187存在的情况下,都能够引起Fyn巯基亚硝基化.结论外源性和内源性NO都能够使Fyn巯基亚硝基化.
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| 关 键 词： | Fyn 巯基亚硝基化 一氧化氮 |
Nitric oxide causes the S-nitrosylation of Fyn tyrosine kinase in vitro |
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| HAO Lingyun,QI Suhua,ZHANG Guangyi. Nitric oxide causes the S-nitrosylation of Fyn tyrosine kinase in vitro[J]. Acta Academiae Medicinae Xuzhou, 2011, 31(11): 718-721 |
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| Authors: | HAO Lingyun QI Suhua ZHANG Guangyi |
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| Affiliation: | ( Research Center for Biochemistry and Molecular Biology, Xuzhou Medical College, Jiangsu Key Laboratory of Brain Disease Bioinformation, Jiangsu Key Laboratory of Anesthesiology, Xuzhou Medical College, Xuzhou, Jiangsu 221002, China) |
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| Abstract: | Objective To investigate the effect of nitric oxide （NO） on the S - nitrosylation of Fyn kinase in vitro. Methods HEK293 cell was transfected with pcDNA3.1 - Fyn plasmid and then was stimulated with S - nitrosoglutathione （GSNO）, or co -transfected with inducible nitric oxide synthase （nNOS） -Fyn plasmid and then was stimulated with A23187 and 7 - nitroindazole （7 - NI）, the S - nitrosylation of Fyn was examined by biotin - switch method and Western blotting. Results Both GSNO and nNOS with A23187 could cause the S -nitrosylation of Fyn. Conclusion Both ectogenic and endogenic NO can cause the S - nitrosylation of Fyn. |
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| Keywords: | Fyn S - nitrosylation nitric oxide |
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