单磷酸阿糖腺苷 病毒唑 柴胡及其联合应用对呼吸道合胞病毒的抑制作用

目的研究单磷酸阿糖腺苷(Ara-AMP)、病毒唑、柴胡及其联合应用对呼吸道合胞病毒(RSV)的抑制作用。方法将Ara-AMP、病毒唑及柴胡分别稀释成不同的浓度,用细胞培养法观察药物的细胞毒性反应;采用Vero细胞先感染病毒2h后给药法观察细胞病变(CPE)和药物对RSV的抑制作用。结果Ara-AMP、病毒唑及柴胡对Vero细胞的毒性剂量(TD)分别为0.25、0.2、2mg/ml;当Ara-AMP、病毒唑分别与柴胡联用时并未增加对细胞的毒性,而Ara-AMP与病毒唑联用时则对细胞的毒性增加;3种药物各自在Vero细胞上对RSV都有抑制作用,Ara-AMP和病毒唑的有效剂量均为25滋g/ml,柴胡为125滋g/ml。当3种药物配伍联合应用时,均可产生协同作用。结论Ara-AMP、病毒唑及柴胡各自对RSV具有显著的抑制作用,它们的联合应用可产生显著协同作用,既可降低用药量,减少毒副反应,又可有效抑制RSV的感染。

Inhibition of respiratory syncytial virus by Ara-AMP,virazole,bupleurum and their joint application

Objective To study the inhibition of respiratory syncytial virus (RSV) by Ara-AMP,virazole,bupleu-rum root injection (bupleurum) and their joint application.Methods Ara-AMP,virazole and bupleurum were diluted to dif-ferent concentrations,and the cell poison reaction by drug cytotoxicity was observed by cell culture;cytopathic effect (CPE) and RSV inhibition by drugs were observed by the way of drug administration two hours after Vero cells were infected by virus. Re-sults The toxic dosages (TD) to Vero cells of Ara-AMP,virazole and bupleurum were 0.25,0.2 and 2 mg/ml,respectively. The drug cytotoxicity was not enhanced when Ara-AMP or virazole combined with bupleurum,but it was done when Ara-AMP combined with virazole.Each of the three drugs inhibited RSV in Vero cells,and the effective dosage of Ara-AMP and virazole was both 25 μg/ml,and bupleurum was 125 μg/ml.The combinations of 3 drugs showed synergism without an-tagonism.After Vero cells were infected by RSV 100 TCID 50 /0.1 ml(ie. median tissue culture infective dose),the results showed RSV was inhibited significantly when Ara-AMP 125 μg/ml,virazole 100 μg/ml and bupleurum 1 000 μg/ml were given to Vero cells tubes in the time of Vero cells just being infected and having been infected for 2 h and 12 h;the inhibition effects were reduced when drugs were given after Vero cells had been infected for 24 h;and no inhibition effects were showed after 36 h.Conclusion RSV could be inhibited by Ara-AMP,virazole and bupleurum,respectively.Significant synergism against RSV was showed by their joint application,this not only can decrease the dosage,toxicity,but also enhance the inhibition ef-fect on RSV obviously.