凋亡相关基因Bcl-2、Bag-1在宫颈上皮病变中的表达及意义

目的：探讨Bcl-2蛋白和Bag-1蛋白在宫颈癌发生、发展中的表达变化以及两者的关系。方法：采用免疫组织化学SP法,分别对39例宫颈上皮内瘤变CINⅠ/Ⅱ、25例CINⅢ（原位癌4例）及36例浸润性宫颈鳞癌（ISCC）组织中的Bcl-2和Bag-1蛋白进行检测,并以20例子宫肌瘤经手术切除的正常宫颈鳞状上皮作对照进行分析。结果：Bcl-2蛋白在正常宫颈组织、CINⅠ/Ⅱ、CINⅢ和宫颈癌组织中表达率分别为10.00%、43.57%、76.00%、66.67%,其表达在CIN和宫颈癌组织间差异无统计学意义（P〉0.05）,在其余各组之间差异有统计学意义（P〈0.05）。Bag-1在正常宫颈组织、CINⅠ/Ⅱ、CINⅢ和宫颈癌组织中的表达率分15.00%、48.72%、72.00%、83.33%,其表达在正常组织与CIN和宫颈癌之间差异有统计学意义（P〈0.01）,在上皮内瘤变与宫颈癌中差异也具有统计学意义（P〈0.05）。宫颈癌中Bcl-2与Bag-1蛋白表达同时增加。结论：宫颈鳞状上皮不典型增生和鳞状上皮癌中均有Bcl-2、Bag-1过表达,表明它们在宫颈癌的发生、发展中均起重要作用。Bcl-2与Bag-1在肿瘤早期抗凋亡作用相互增强,呈正相关关系。

Apoptosis-regulating gene Bcl-2 and Bag-1 expression in cervical carcinogenesis

Objective:To investigate the expression of Bcl-2,Bag-1 in the pathogenesis and progression of cervical cancer and their relationship.Methods:S-P immunohistochemical analysis was performed in 36 cervical carcinoma and 64 cases of CIN.Results:Overexpression of Bcl-2 and Bag-1 protein in CIN and cervical cancer was remarkably higher than that in the control(P>0.01),whereas the difference between CIN and cervical cancer was not significant(P>0.05).Bcl-2 protein expression was positive correlation with Bag-1 protein expression in cervical carcinoma.Conclusion:Bag-1 and Bcl-2 overexpression might play an important role in the pathogenesis and development of cervical cancer.The overexpressions of Bcl-2 and Bag-1 are the early event of cervical carcinogenesis which are associated with the development of cervical carcinoma.Bcl-2 and Bag-1 protein in the development of cervical carcinoma enhance each other.