CDH1基因甲基化与食管鳞癌的关系

目的 探对食管鳞癌组织中CDH1基因启动子区甲基化的表达状况及其与烟酒史、肿瘤家族史的关系.方法 应用甲基化特异性聚合酶链反应(MSP)法检测食管鳞癌中CDH1基因启动子区甲基化情况.结果 食管鳞癌、癌旁及正常组织CDH1基因的甲基化阳性率分别为51.6％(32/62)、22.6％(14/62)和0(0/18).癌与癌旁组织比较,甲基化阳性率差异有统计学意义(P＜0.01)；癌和正常组织比较,甲基化阳性率差异有统计学意义(x2=15.484,P＜0.01)；癌旁与正常组织间差异无统计学意义(x2 =3.487,P＞0.05).食管鳞癌CDH1基因甲基化情况与其性别、年龄、烟酒史和上消化道肿瘤家族史比较差异均无统计学意义(均P ＞0.05).结论 CDH1基因启动子区甲基化与食管鳞癌发生关系密切,在食管鳞癌中是一常见的表观遗传学事件.CDH1基因甲基化可能是一个独立的危险因素.

Relationship between CDH1 gene methylation and esophageal squamous cell carcinoma

Objective To study CDH1 gene promoter methylation status in tissues of esophageal squamous cell carcinomas（ESCC） and its relationship with tobacco and alcohol history,family history of cancer.Methods CDH1 gene promoter methylation in ESCC was detected by methylation-specific polymerase chain reaction（MSP） and was analyzed statistically.Results CDH1 gene methylation positive rates in tumor tissues,adjacent and normal tissues of ESCC were 51.6%（32/62）,22.6%（14/62） and 0（0/18）,respectively,the difference between cancer and adjacent tissue was significant（P0.01）;there was significant difference between tumor tissues and normal tissues（χ2=15.484,P0.01）;difference between adjacent tissues of ESCC and normal tissues was no statistical significance（χ2=3.487,P0.05）.Compared CDH1 gene methylation in ESCC with sex,age,tobacco and alcohol history,family history of upper gastrointestinal tumors,there were no statistical significance（all P0.05）.Conclusion CDH1 gene promoter methylation is closely related to the occurrence of ESCC,and it is a common epigenetic event in ESCC.CDH1 gene methylation may be an independent risk factor.