丹参水溶性部位抑制血管内皮细胞凋亡的作用及机制研究

目的：探明丹参水溶性部位对脂多糖（LPS）处理的血管内皮细胞（VEC）凋亡的保护作用及机制。方法：内毒素处理培养的人脐静脉内皮细胞造成细胞凋亡。培养液中添加系列浓度的丹参水溶性成分培养细胞,倒置相差显微镜观测细胞形态变化。吖啶橙染色,共聚焦显微镜观测凋亡细胞核片段化状况;PI染核,流式细胞仪检测细胞周期分布。生化试剂盒检测细胞内丙二醛（MDA）含量及超氧化物歧化酶（SOD）、谷胱甘肽过氧化物酶（GSH-PX）活性。结果：脂多糖处理使VEC形成大量凋亡小体,细胞核片段化严重,明显降低了细胞存活率,改变了细胞周期的正常分布,阻止细胞由G1期进入S期。同时显著上调了细胞中的MDA含量,明显下调了其中的SOD、GSH-PX活性。丹参水溶性部位在一定浓度范围内,剂量依赖性地逆转脂多糖引起的上述变化。结论：丹参水溶性部位可能通过调节细胞氧化应激状态抑制脂多糖导致的VEC凋亡。

Studies on the effect and the underlying mechnism of aqua-soluble extract of Salvia miltiorrhiza Bge. inhibits apoptosis in vascular endothelial cells

Objective：To find out the role of aqua-soluble extract of Salvia miltiorrhiza Bge. in the protection of vascular endothelial cells（VEC） and its possible mechanism in vitro. Methods：Added a series of this ingredient to human umbilical vein endothelial cells （HUVEC） treated with lipopolysaccharide （LPS）. Apoptotic morphological changes of cells were observed under inverted phase contrast microscope. Nuclear fragmentation of cells were ob- served under laser scanning confocal microcopy after being stained with acridinorange. Cell cycle distribution was detected by the flow-cytometric after being stained with PI. The activities of superoxide dismutase（SOD） and glutathione peroxidase（GPH-PX） as well as maleic dialdehyde（MDA） level in cells were measured by spectrophotometfie methods as described in the assay kits. Results ：Apoptotic morphological changes and the decrease of cell viability of these cells were obviously inhibited by aqua-soluble extract of Salvia miltiorrhiza Bge. in a dose-dependent way. Furthermore,the abnormal cell cycle distribution,the decrease of GSH-Px activity and the increase of MDA level induced by LPS were markedly reversed. Conclution：The aqua-soluble extract of Salvia miltiorrhiza Bge. produces protective action on VEC induced by LPS via an antioxidative mechanism.