蕨麻对缺氧诱导心肌细胞凋亡的影响

目的观察蕨麻(Potentilla anserina L.)对缺氧诱发乳鼠心肌细胞凋亡的影响,并探讨其可能机制。方法采用原代培养的SD乳鼠心肌细胞建立缺氧损伤实验模型,分为正常对照组、缺氧模型组、蕨麻醇提物干预组,通过HE染色、双苯并咪唑-碘化丙锭荧光染色及透射电镜技术,观察细胞镜下结构的改变,并通过免疫组织化学染色观察细胞内P53蛋白表达水平。结果与缺氧模型组和正常对照组比较,蕨麻醇提物干预组可有效减轻缺氧导致的心肌细胞肿胀变形、细胞质空泡化及DNA凝集等损伤变化,并可显著降低P53蛋白的表达(P<0.01)。结论蕨麻能有效抑制缺氧诱导的心肌细胞凋亡损伤,其机制之一可能是通过调节P53蛋白的表达实现的。

Effect of Potentilla anserina L. on cardiomyocyte apoptosis induced by hypoxia

Objective To study the effect of Potentilla anserina L.on apoptosis of cultured neonatal rat cardiomyocytes induced by hypoxia and its possible mechanism.Methods A model of hypoxia injury was established by using primary cultured myocytes of neonatal SD rats.Using HE staining,Hochest-PI staining and technique of transmission electron microscopy(TEM),the morphological changes of cardiac myocytes were observed.The mechanism was studied using immunohistochemical staining of P53 protein at molecular biological level.Results Compared with hypoxic model group and normal control group,the extract of Potentilla anserina L.in alcohol could significantly attenuate the injurious changes,such as swelling,fragmentation and condensation of nucleus and could downregulate the expression of P53 protein(P<0.01).Conclusion Potentilla anserina L.has a notable attenuating effect on apoptosis of cardiomyocytes induced by hypoxia.This effect might be related to regulating the expression of P53 protein.