低氧对小鼠骨髓间质干细胞增殖及黏附的影响

目的 观察低氧环境对小鼠骨髓间充质干细胞(MSCs)增殖、黏附及分泌的影响.方法 采用细胞增殖实验、细胞黏附实验及Western blot分别观察小鼠MSCs在低氧(6%O2)和常氧(21%O2)的增殖、黏附,及纤连蛋白(FN)和玻璃粘连蛋白(VN)的表达.结果 低氧组10d内培养即可扩增到(1.20±0.19)×105细胞/孔,明显高于常氧组(0.65±0.08)×105细胞/孔(P<0.05);低氧组24 h细胞黏附率(88.64±1.4)%高于常氧组(60.83±1.6)%(P<0.05),同时FN和VN的蛋白表达显著增加.结论 低氧作为体外的一种可控制因素,调节MSCs的增殖及黏附功能,对于MSCs的组织工程的应用具有一定的意义.

Effects of low oxygen tension on the proliferation and adhesion of mouse MSCs

Objective To investigate the effects of low oxygen tension on the proliferation, adhe-sion and secretion of mouse mesenchymal stem ceils (MSCs). Methods Proliferation test, adhesion as-say, and Western blot were performed to observe proliferation, adhesion and the protein expression of fi-branectin (FN) and vitronectin (VN) of mouse MSCs cultured in 6% O2 and 21% O2 Results Com-pared to 21% O2, (1.2±0.19) × 105 cells/well could be obtained after 10 days culture in 6% O2 (P <0.05). Ratio of 24 h adhesion in 6% O2 group [(88.64±1.4) %] was significantly higher than that in 21% O2 group [(60.83±1.6) %] (P < 0.05). The protein expression of FN and VN were also higher in 6% O2 group. Conclusion Low oxygen tensian,as an in vitro factor which could be controlled,can regu-late MSCs in proliferation, adhesion and extracellular matrix secretion,which plays a role in the application of MSCs in tissue engineering.