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Stress hormone stability: processing of blood samples collected during parabolic flight. A pre-flight comparison of different protocols
Authors:Schneider Stefan  Brümmer Vera  Carnahan Heather  Dubrowski Adam  Askew Christopher D  Strüder Heiko K
Affiliation:1. Department of Otolaryngology, Head and Neck Surgery, Northwestern University, Chicago, IL, USA;2. Division of Otolaryngology, Head and Neck Surgery, Cook County Hospital (Stroger Hospital), Chicago, IL, USA;1. Center for BrainHealth, The University of Texas at Dallas, Dallas, TX, USA;2. Callier Center for Communication Disorders, The University of Texas at Dallas, TX, USA;3. Medical Scientist Training Program, Washington University in St. Louis, MO, USA;4. Department of Neurology and Neurotherapeutics, The University of Texas Southwestern Medical Center, Dallas, TX, USA;5. Department of Radiology, The Johns Hopkins University School of Medicine, Baltimore, MD, USA
Abstract:OBJECTIVES: Compare different protocols for blood processing to be used during parabolic flights. DESIGN AND METHODS: Measurement of cortisol (COR), prolactin (PRL), adrenocorticotropic hormone (ACTH), epinephrine (EP) and norepinephrine (NOR) concentrations stored at different temperatures and intervals before analysis. RESULTS: COR, PRL and NOR showed no changes in concentration between analysis protocols. ACTH dropped by 60% when not analysed within 24 h. CONCLUSION: A standardised processing protocol that includes a 4-h delay following blood collection is suitable for the assessment of serum stress hormone concentrations.
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