淫羊藿总黄酮对成骨细胞中OPG和RANKL mRNA基因表达影响的实验研究

目的:探讨淫羊藿总黄酮对大鼠成骨细胞中骨保护素(OPG)和核因子κB受体激活因子配体(RANKL) mRNA基因表达的影响.方法:体外培养成骨细胞,分别加入含0.1、1、10、100 ng/ml淫羊藿总黄酮(HEF)的培养液,48 h及96 h后分别提取细胞总mRNA,用RT-PCR方法分析OPG/RANKL的mRNA的表达,进行半定量分析.结果:培养48 h后,与对照组比较,HEF增强了OPG mRNA的表达,有显著性差异(P＜0.05或P＜0.01),VOPG/VRANKL比值增大;96 h后,HEF明显增强了OPG mRNA的表达,各浓度组与对照组相比,均有显著性差异(P＜0.05或P＜0.01),各浓度组VOPG/VRANKL比值增大,有显著性差异(P＜0.05或P＜0.01).结论:淫羊藿总黄酮可能通过增加成骨细胞OPG的表达来抑制破骨细胞的分化和成熟,从而抑制骨吸收,达到治疗骨质疏松症的目的.

Effect of Herba Epimedii Flavone on Expression of OPG and RANKL in Rat Osteoblasts

Objective: To explore the effect of Herba Epimedii Flavone(HEF) on expression of OPG and RANKL in rat osteoblasts. Methods: Osteoblasts were obtained from new born rat calvaria by digestive enzymes. The different HEF concentrations(0.1,1,10,100 ng/ml)were added into the culture medium. Total mRNA was prepared from osteoblasts after 48 h and 96 h, and mRNA expression of OPG and RANKL were detected by RT-PCR . Results: The mRNA expression of OPG in osteoblasts significantly increased compared with that of the control group, and the V_OPG/V_RANKL ratio was deceased markedly in the treatment of 10 ng/ml group after 48 h. After 96 h, the mRNA expression of OPG significantly increased and the V_OPG/V_RANKL ratio was also decreased in the treatment group compared with that of the control group. Conclusion: HEF suppress the differentiation and maturate of osteoblast. Osteoporosis can be prevented and cured by HEF.