BMP-4基因/TGF-β1对兔火器性股骨缺损的治疗作用

目的 观察BMP-4基因联合TGF-β1对火器性股骨缺损的修复作用. 方法 用火器性钢珠造成兔股骨中段缺损,伤后2周于骨缺损上、下端和中部注射经体外鉴定的BMP-4基因和TGF-β1.用RT-PCR和Western blot检测BMP-4 mRNA与蛋白在体内的表达,用碱性磷酸酶(ALP)活性和钙含量检测局部组织的成骨能力,用病理学和X线检查成骨过程和质量. 结果 注射后BMP-4 mRNA和蛋白可在体内表达6周.实验组ALP活性伤后8周时增加到(13.17±0.51)U/100 ml,显著高于对照组的(8.77±0.44)U/100 ml,提示实验组的成骨能力增强,钙含量检测与此相符.病理学和X线检查证明实验组成骨过程加快,成骨质量提高. 结论 BMP-4基因/TGF-β1对火器性骨缺损有明显的促修复作用.

Gene therapy of firearm femur defect by using BMP-4/TGF-β1 in rabbits

Objective To observe effects of BMP-4 gene combined with TGF-β1 in repairing rabbit femur defect resulted from firearm wound. Methods The femur defect was made with firearm steel ball. Plasmid encoded BMP-4 gene identified in vitro and TGF-β1 were injected into the tissue of upper, lower and central parts of the defects at the second week after wound. The mRNA and protein expressions of BMP-4 in vivo were detected by real time-polymerase chain reaction (RT-PCR) and Western blot. Activity of alkaline phosphatase (ALP) and calcium content were measured for evaluating osteogenetic ability. The process and quality of osteogenesis were determined by pathological and X-ray examinations. Results mRNA and protein of BMP-4 could continually express for six weeks in vivo after injection. Activity of ALP in the experimental group was increased to ( 13.17 ±0.51 ) U/100 ml at the 8th week, which was significantly higher than (8.77 ± 0.44) U/100 ml in the control group, indicating that osteogenetic ability was markedly enhanced, which accorded with determination of calcium content. Pathological observation and X-ray proved prominent improvement of osteogenesis, with a shorter time and better quality, in the experimental group. Conclusion BMP-4/TGF-β1 can promote repair of firearm femur defect.