p16基因甲基化与156例广西巴马壮族老人长寿的关系

目的探讨p16基因甲基化与人类长寿的关系。方法应用甲基化特异性PCR技术对巴马156例(年龄90～105岁,平均93.2岁,长寿组)健康壮族长寿老人和142例健康壮族成年人(年龄20～72岁,平均34.8岁,对照组)的p16基因甲基化状况进行分析,分析该基因甲基化在长寿组和对照组之间的差异及其与长寿的关系。结果长寿组p16基因甲基化明显低于对照组(P0.05)。结论巴马壮族长寿老人p16基因甲基化程度明显降低。

Relationship of methylation of p16 gene with long life expectancy of Zhuang ethnic group elderly people:report of 156 cases in Guangxi Bama area

Objective To explore the relationship between the methylation of p16 gene and human longevity. Methods The methylation specific polymerase chain reaction was used to analyze the methylation status of p16 gene in 156 long-lived healthy Zhuang ethnic group people （aged 90-105 years, mean 93. 2 years, long-lived group, LG） and 142 healthy controls （aged 20-72 years,mean 34. 8 years, control group,CG） from Bama area. The difference in p16 gene methylation between the two groups was analyzed to study the role of p16 gene methylation in human longevity. Results The methylation of p16 gene was more frequent in CG than in LG. Conclusion The methylation of p16 gene is less frequent in long-lived Zhuang ethnic group elderly people in Bama area.