长期游泳训练和补充大豆多肽对高脂饮食大鼠脂肪肝形成过程中脂代谢的影响

目的:研究长期游泳训练和补充大豆多肽对高脂饮食大鼠脂肪肝形成的干预作用及其机制。方法:雄性SD大鼠60只,随机分为C组(普通饲料对照组)、HF组(高脂饮食组)、HE组(高脂饮食 运动组)、HS组(高脂饮食 大豆多肽组)、HES组(高脂饮食 运动 大豆多肽组)5组,HE组和HES组大鼠进行每周6次、每次60min的无负重游泳训练,HS组和HES组大鼠按500mg/kgBW的剂量补充大豆多肽。10周后观察大鼠肝脏的形态结构,并检测大鼠血清甘油三酯(TG)、总胆固醇(TC)和肝内TG、TC以及游离脂肪酸(FFA)含量。结果:(1)HF组大鼠肝脏出现严重的大泡性脂肪变性,并有不同程度的炎性细胞浸润,且血清TG(P<0.05)、TC(P<0.01)和肝内TG(P<0.05)、TC(P<0.01)以及FFA(P<0.01)含量显著高于对照组。(2)与HF组相比,HE、HS和HES组大鼠肝脂肪变性显著减轻。双因素方差分析表明,有氧游泳训练使高脂饮食大鼠肝TG、TC含量虽有降低,但不显著;而使血清TG(P<0.01)、TC(P<0.01)以及肝FFA含量(P<0.01)显著降低。补充大豆多肽可使高脂饮食大鼠血清、肝TG、TC以及肝内FFA含量(P<0.01)显著降低。有氧游泳训练联合补充大豆多肽对降低高脂饮食大鼠血清TG(P<0.01)、TC(P<0.05)和肝TG(P<0.01)、TC(P<0.01)、FFA(P<0.01)含量具有显著的交互作用。结论:(1)10周的高脂饮食可以诱导大鼠脂肪肝的形成,且与脂肪代谢紊乱有关。(2)有氧运动和补充大豆多肽可通过改善高脂饮食大鼠的脂肪代谢预防脂肪肝的形成。

Effect of Long-term Swimming and Supplementation of Soybean Polypeptide on Lipids Metabolism during the Formation of Fatty Liver in High-fat-diet Rats

Objective To explore the effect of aerobic exercise and the supplement of soybean polypeptide on the formation Fatty liver disease in high-fat-diet SD rats.Methods Sixty male SD rats were randomly divided into five groups: control group(C),high fat diet group(HF),high fat diet exercise group(HE),high fat diet soybean polypeptides group(HS),high fat diet exercise soybean polypeptides group(HES).Except the rats in control group,the rats in other groups were fed with high fat diets to induce fatty liver disease.Swimming training(60min per time,6 times per week) and /or soybean polypeptides supplementation(500mg/kgBW) was intervened for ten weeks.The morphological changes of the liver tissues was observed under microscope,and serum triacylglycerols(TG),total cholesterol(TC) and liver TG,TC,free fatty acid(FFA) concentration were detected at the end of the experiment.Results(1)Compared with C group,severe macrovesicular steatosis and inflammation were observed in HF group,and serum TG(P<0.05),TC(P<0.01) and TG(P<0.05),TC(P<0.05),FFA(P<0.01) in the liver increased significantly.(2)Compared with HF group,liver steatosis in HE,HS and HES groups was attenuated significantly.By double factor variance analysis,aerobic swimming training could decrease liver TG and TC concentration in high-fat-diet rats,but no significant difference was found.While it could decrease serum TG(P<0.01),TC(P<0.01) and liver FFA(P<0.01) concentration significantly.Soybean polypeptides supplementation could decrease serum and liver TG,TC and liver FFA(P<0.01) concentration in high-fat-diet rats significantly.Aerobic swimming training combined with soybean polypeptides supplementation had a significant co-action on reducing serum TG(P<0.01),TC(P<0.05) and liver TG(P<0.01),TC(P<0.01),FFA(P<0.01) concentration in high-fat-diet rats.Conclusions(1)10-week high fat diets might cause the formation of fatty liver,which was related to the disorder of lipids metabolism.(2)Aerobic exercise training and the supplement of soybean polypeptides could prevent the formation of fatty liver in high-fat-diet rats by improving lipids metabolism.