| Bcl-2反义核酸对人胆管癌QBC939细胞增殖的影响 |
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| 引用本文： | 彭创,汤恢焕,黄平,陈明雄,石永忠. Bcl-2反义核酸对人胆管癌QBC939细胞增殖的影响[J]. 实用癌症杂志, 2005, 20(4): 355-357 |
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| 作者姓名： | 彭创 汤恢焕 黄平 陈明雄 石永忠 |
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| 作者单位： | 410008,中南大学湘雅医院普外科 |
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| 基金项目： | 湖南省卫生厅科研基金资助项目,NoB2004.168 |
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| 摘 要： | 目的研究Bcl-2反义核酸（ASODN）对人胆管癌QBC939细胞增殖的影响。方法将Bcl-2ASODN与QBC939细胞共同孵育，采用台盼蓝拒染实验检测细胞存活率，采用克隆形成实验俭测克隆形成率。结果台盼蓝拒染实验和克隆形成实验检均显示Bcl-2ASODN可以部分抑制QBC939细胞增殖，经ASODN作用细胞的存活率和克隆形成率均显著低于对照组（P〈0．05）。结论Bcl-2反义核酸对人胆管癌QBC939细胞增殖有抑制作用。
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| 关 键 词： | 胆管肿瘤 Bcl-2基因 寡脱氧核苷酸类 |
| 文章编号： | 1001-5930（2005）04-0355-03 |
| 收稿时间： | 2005-02-10 |
| 修稿时间： | 2005-06-18 |
Effect of Bcl-2 Antisense Oligodeoxynucleotides on Cell Proliferation in Bile Duct Carcinoma Cell Line QBC939 |
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| PENG Chuang , TANG Hai-huan , HUAN Piing,et al.. Effect of Bcl-2 Antisense Oligodeoxynucleotides on Cell Proliferation in Bile Duct Carcinoma Cell Line QBC939[J]. The Practical Journal of Cancer, 2005, 20(4): 355-357 |
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| Authors: | PENG Chuang TANG Hai-huan HUAN Piing et al. |
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| Affiliation: | Department of General Surgery, Xiang Ya Hospital, Central South University , Changsha , 410008 |
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| Abstract: | Objective To investigate the effect of Bcl-2 antisense oligodeoxynucleotides(ASODN) on cell proliferation in bile duct carcinoma cell line QBC939.Methods Survival rate and colony formation rate of QBC939 cells incubated with Bcl-2 ASODN were evaluated by trypan blue staining assay and colony forming test.Results Both trypan blue staining assay and colony forming test demonstrated that Bcl-2 ASODN could partially inhibit the growth of QBC939 cells.After incubated with Bcl-2 ASODN,The survival rate and colony formation rate of QBC939 cells exhibited significant lower than controls(P<0.05).Conclusion Bcl-2 ASODN inhibited cell proliferation in bile duct carcinoma cell line QBC939. |
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| Keywords: | Bile duct neoplasms Bcl-2 gene Oligodeoxynucleotides |
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