SELDI蛋白芯片技术检测叶酸对胎鼠神经干细胞作用的蛋白表达谱

目的 探讨应用表面增强激光解离飞行时间质谱技术(surface-enhanced laser desorption/ionization time of flight mass spectrometry,SELDI-TOF-MS)测试不同剂童叶酸作用下胎鼠神经干细胞(NSCs)的蛋白质表达谱,从中筛选出差异表达蛋白.方...

DETECTION OF THE PROTEINS PROFILE OF FETAL NEURAL STEM CELLS TREATED WITH FOLIC ACID BY SELDI PROTEIN CHIP TECHNOLOGY IN RAT

Objective To detect the fetal rat neural stem cell(NSC) protein profiling treated with different doses of folic acid(FA) by surface-enhanced laser desorption/ionization time of flight mass spectrometry(SELDI-TOF-MS) and find out the differential proteins.Method Primary cultured fetal rat NSC were divided into 4 groups: control group(FA 4μg/ml in media),FA-deficient group(FA 0.65μg/ml in media),low FA group(FA 8μg/ml in media),high FA group(FA 44μg/ml in media).NSC proliferation was detected after treatment of FA by methylthiazoltatragolium(MTT) technique.Wcx-2 chip was used for discovering the discriminatory proteins,and the results were analyzed by Biomarker Wizard software.Differential proteins were initially identified through querying the protein database.Results MTT assay showed that the growth tendency was increased with folate concentration in the media.102 proteins were differentially expressed on the chips.Compared with the control group,32 proteins were differentially expressed in other groups,and 4 proteins of them had significant differences(P<0.05).These proteins were primarily considered to be associated with apoptosis in NSCs,and expressed differently in protein atlas of different FA groups.Conclusion Folic acid may alter the expression of apoptic protein during the NSCs growth.The effect of FA in prevention and treatment of nervous system diseases will be further explored.