Low androgen status inhibits erectile function by up-regulating the expression of P2X receptors in rat corpus cavernosum |
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Authors: | Chuan Guo Jun Jiang Bo Cheng Libo Xie Haocheng Lin Rui Jiang |
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Institution: | 1. Department of Urology, The Affiliated Hospital of Southwest Medical University, Luzhou, China
Department of Urology, Chengfei Hospital, Chengdu, China;2. Department of Thyroid Surgery, The Affiliated Hospital of Southwest Medical University, Luzhou, China;3. Department of Urology, The Affiliated Hospital of Southwest Medical University, Luzhou, China;4. Department of Urology, Peking University Third Hospital, Beijing, China |
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Abstract: | The aim of our study was to investigate whether low androgen level inhibits the erectile function of rats by regulating the expression of P2X receptors. Thirty-six 8-week-old male SD rats were randomly divided into six groups: sham-operated groups (4w-sham, 8w-sham), castration groups (4w-cast, 8w-cast) and androgen replacement after castration groups (4w-cast + T, 8w-cast + T). The maximum intracavernous pressure/mean arterial pressure (ICPmax/MAP), the levels of serum testosterone (T) and nitric oxide (NO), and the expression of P2X1, P2X2, P2X3, eNOS, p-eNOS, ROCK1 and ROCK2 in the cavernous tissue of rats were determined. The serum T, ICPmax/MAP and NO levels in penile corpus cavernosum in the castration groups were significantly lower than those in other groups (p < .01). The protein expression of P2X1, P2X2, P2X3, ROCK1 and ROCK2 in the castration groups was significantly higher than those in other groups (p < .01). P-eNOS/eNOS of the castration groups were significantly lower than those of other groups (p < .01). The serum T level was negatively correlated with the expression of P2X1, P2X2 and P2X3 in the corpus cavernosum. Low androgen level inhibits erectile function by up-regulating the expression of P2X1, P2X2, P2X3 and RhoA/Rho-kinase resulting in reducing the ratio of p-eNOS/eNOS and the level of NO in corpus cavernosum of rats. |
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Keywords: | androgen endothelial nitric oxide oxygenase erectile dysfunction P2X receptor RhoA/Rho-kinase |
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