IFN-α抑制HUVECs以及荷人肝癌裸小鼠血管生成的实验研究

目的: 检测IFN-α体内外对人脐静脉血管内皮细胞（HUVECs）的抑制作用，探讨IFN-α抑制肝癌血管生成的机制。方法： 运用细胞增殖抑制试验、MTT试验、管样结构形成试验、移动抑制试验以及裸鼠体内移植瘤生长和血管密度测定探讨IFN-α对HUVECs的抑制作用。结果： IFN-α对HUVECs的细胞增殖、移动和管样结构形成试验有明显的抑制作用，体内实验提示试验组移植瘤直径明显小于对照组，微血管密度（MVD）亦明显低于对照组。结论： IFN-α可以通过抑制肿瘤的血管新生，起到抑制肿瘤生长的作用。

Anti- angiogenesis effects of IFN- α on cultured HUVECs and human HCC bearing nude mouse

AIM:To evaluate the anti-angiogenesis effect of interferon-α (IFN-α) on cultured human umbilical vein endothelial cells(HUVECs) and human hepatocellular carcinoma (HCC) bearing nude mouse.METHODS:Anti-proliferation test,MTT test，tube-formation test,migration test on cultured HUVECs were employed and the tumor volume and microvascular desity (MVD) of IFN-α treated human hepatoma cell line (HuH7) were meassured. RESULTS:IFN-α displayed apparent inhibitory effects on cultured HUVECs in anti-proliferation test,MTT test,tube-formation test,migration test,and the tumor diameter and MVD in IFN-α treated HuH7 inoculated nude mouse group were significantly less than those in PBS treated HuH7 inoculated nude mouse group.CONCLUSION: IFN-α inhibits tumor growth through anti-angiogenesis.