| 三苯氧胺和雌激素对MCF-7乳癌细胞内耐药基因mdr-1mRNA的影响 |
| |
| 引用本文： | 郑军,姚榛祥,邓兵. 三苯氧胺和雌激素对MCF-7乳癌细胞内耐药基因mdr-1mRNA的影响[J]. 重庆医科大学学报, 2000, 25(3): 245-246 |
| |
| 作者姓名： | 郑军 姚榛祥 邓兵 |
| |
| 作者单位： | 1. 重庆医科大学临床学院普外科 400016
2. 重庆医科大学儿科学院免疫实验室 400010 |
| |
| 摘 要： | 目的 观察三苯氧胺（ａｍｏｘｉｆｅｎ，ＴＡＭ）及雌激素（Ｅ２）对乳腺癌ＭＣＦ－７细胞株耐药基因ｍｄｒ－１ｍＲＮＡ水平的影响：方法 应用ＲＴ－ＰＣＲ法观察ＴＡＭ及Ｅ２在不同时间、浓度作用下细胞内ｍｄｒ－１ｍＲＮＡ水平的变化；结果ＴＡＭ可以使细胞内ｍｄｒ－１ｍＲＮＡ水平降低，而Ｅ２则相反。且以上变化表现出剂量、时间依赖关系。结论 ＴＡＭ通过降低ｍｄｒ－１ｍＲＮＡ水平，使肿瘤细胞的耐药性降低，有利于抗肿
|
| 关 键 词： | mdr－1mRNA 乳腺癌 三苯氧胺 雌激素 |
| 文章编号： | 0253-3626(2000)03-0245-02 |
| 修稿时间： | 1999-12-06 |
The effect of estrogen and TAM on the mdr-1 mRNA level in MCF-7 cell by RT-PCR |
| |
| ZHENG Jun,et al. The effect of estrogen and TAM on the mdr-1 mRNA level in MCF-7 cell by RT-PCR[J]. Journal of Chongqing Medical University, 2000, 25(3): 245-246 |
| |
| Authors: | ZHENG Jun et al |
| |
| Affiliation: | ZHENG Jun,et al Department of General Surgery,College of Clinical Medicine |
| |
| Abstract: | Objective Study the effects of Tamoxifen(TAM) and estrogen(E2) on the mdr-1 mRNA level in MCF -7 cell. Methods RT-PCR was used to observe the variation of mdr-1 mANA level in MCF-7 cell after treatment with TAM and E2 at different time and concentration. Results TAM could decrease the level of mdr-1 mRNA in MCF-7 cell but E2 could not. These results came together with the relationship with the concentration of the drug and the treatment of the given time. Conclusion The drug-resistance of neoplasm cells could be down -regulared by TAM via decreasing the mRNA level in cells, which would be helpful in tumor drug therapy. |
| |
| Keywords: | TAM E2 Breast cancer cells mdr-1 |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
