| 首乌藤多糖酶法提取工艺研究 |
| |
| 引用本文： | 董晶晶,李晓坤,杨云,许闽.首乌藤多糖酶法提取工艺研究[J].中国现代应用药学,2011,28(6):519-523. |
| |
| 作者姓名： | 董晶晶 李晓坤 杨云 许闽 |
| |
| 作者单位： | 河南中医学院药学院，郑州 450008;河南中医学院药学院，郑州 450008;河南中医学院药学院，郑州 450008;河南中医学院药学院，郑州 450008 |
| |
| 基金项目： | 河南省郑州市金水区科技攻关项目(20093543) |
| |
| 摘 要： | 目的研究酶法提取首乌藤多糖的最佳工艺条件。方法利用酶解法提取首乌藤多糖,筛选出最佳适用酶,然后利用苯酚-硫酸法测定多糖含量,以多糖得率和多糖含量为综合评价指标,采用单因素试验对影响首乌藤多糖的提取工艺进行研究。结果首乌藤多糖的最佳适用酶为纤维素酶,由单因素试验确定的酶法提取首乌藤多糖的最佳工艺条件为pH 5.0,纤维素酶用量3%,酶解时间4 h,酶解温度55℃。结论纤维素酶可显著提高首乌藤多糖的提取率。
|
| 关 键 词： | 首乌藤多糖 酶法 提取工艺 |
| 收稿时间： | 2010/7/21 0:00:00 |
Study on Extraction Process of Polysaccharides form Polygoni Multiflori Caulis by Enzyme Method |
| |
| DONG Jingjing,LI Xiaokun,YANG Yun and XU Min.Study on Extraction Process of Polysaccharides form Polygoni Multiflori Caulis by Enzyme Method[J].The Chinese Journal of Modern Applied Pharmacy,2011,28(6):519-523. |
| |
| Authors: | DONG Jingjing LI Xiaokun YANG Yun and XU Min |
| |
| Institution: | College of Pharmacy, Henan University of Traditional Chinese Medicine, Zhengzhou 450008, China;College of Pharmacy, Henan University of Traditional Chinese Medicine, Zhengzhou 450008, China;College of Pharmacy, Henan University of Traditional Chinese Medicine, Zhengzhou 450008, China;College of Pharmacy, Henan University of Traditional Chinese Medicine, Zhengzhou 450008, China |
| |
| Abstract: | OBJECTIVE To study the optimal enzyme extraction technology of polysaccharides from Polygoni Multiflori Caulis.METHODS The yield of polysaccharide and polysaccharide content were used to evaluate the factor levels.Based on the optimized enzyme,the extraction conditions were optimized by the single factor tests.RESULTS The conditions of the optimal extraction process using cellulose enzyme method were as follows: the pH value was 5.0,the concentration of cellulose enzyme was 3%,the extraction time was 4 hour... |
| |
| Keywords: | Polygoni Multiflori Caulis polysaccharide enzyme method extraction process |
| 本文献已被 CNKI 维普 等数据库收录！ |
| 点击此处可从《中国现代应用药学》浏览原始摘要信息 |
| 点击此处可从《中国现代应用药学》下载免费的PDF全文 |
|
