HPLC法考察人工栽培草珊瑚中异嗪皮啶,迷迭香酸和落新妇苷含量

目的考察不同栽培条件草珊瑚中异嗪皮啶,迷迭香酸和落新妇苷含量。方法样品来自不同栽培条件下的草珊瑚。采用HPLC测定异嗪皮啶、迷迭香酸和落新妇苷。色谱柱:ZORBAX SB-C18(250 mm×4.6 mm,5μm);流动相:A:乙腈-0.1%磷酸溶液(20:80),B:乙腈-0.1%磷酸溶液(90:10),由A到B梯度洗脱;流速:1 ml/min;检测波长:330、290nm。结果各组分含量与不同栽培条件有关。不同部位各组分含量差别显著。结论采用异嗪皮啶,迷迭香酸和落新妇苷进行多指标含量测定,能较全面反映草珊瑚品质;本实验区草珊瑚品质优良;地上部位有较高的药用价值。

Content determination of isofraxidin, rosmarinic acid and astilbin from Herba Sarcandrae in different artifical cultivated

Objective To determination isofraxidin, rosmarinic acid and astilbin content in different artifical cultivated Herba Sarcandrae. Methods Test sample were classified by plant ports of above and below ground ,and determine by HPLC. A Zorbax SB-C₁₈ column was used, with the mobile phase of CH₃CN-0.1%H₃PO₄ (20:80)and CH₃CN-0.1%H₃PO₄(90:10). The gradient elution; detection wavelength was 330 nm and 290nm. Results The content of isofraxidin, rosmarinic acid and astilbin content in different artifical cultivated Herba Sarcandrae and different parts of Sarcandrae were quite different. Conclusion Determination of isofraxidin, rosmarinic acid and astilbin content ,could fully reflect the quality of Sarcandrae. The experimental area of artifical cultivated Sarcandrae have good quality; plant part above ground have a high medicinal value.