硝酸甘油对大鼠重症急性胰腺炎ET／NO、TXA2／PGI2的影响

目的探讨硝酸甘油对大鼠重症急性胰腺炎（SAP）ET／NO、TXA2／PGI2和胰腺病理形态学的影响。方法SD大鼠60只分为5组：A正常胰腺组，B、C、D和E组为实验组，胰胆管逆行注射5％牛磺胆酸钠制作SAP模型。C、D和E组分别静脉注射硝酸甘油0．5、1．0、2．0μg/（kg·min），A、B纽注射生理盐水。6h及12h后分别检测各组ET、NO、TXB2及6-keto—PGFIa值并进行胰腺病理标本评分。结果6h及12h时的标本数据分别显示，与B组比较，C、D和E组的胰腺病理评分、ET、ET／NO、TXB2、TXB2／6-keto—PGFIa比值都降低（P〈0．05）。结论硝酸甘油具有降低大鼠血液ET、ET／NO、TXA2、TXA2／PGI2值的作用，改善胰腺血液微循环，延缓大鼠重症急性胰腺炎的炎性病理变化的作用。

The effect of nitriglycerin on ET/NO and TXA2/PGI2 in severe acute pancreatitis rats

Objective To explore the effect of nitroglycerin on ET/NO, TXA2/PGI2 and pancreas pathomorphology changes in severe acute pancreatitis (SAP) rats. Methods Sixty SD rats were random divided into five groups, including control group (A group, n = 12) and experimental group(B,C,D and E group, n = 12). The SAP was induced by injection of 5% sodium taurocholate through retrograde common biliopancreatic ducts via duodenal papilla with epidural catheter. Group C, D and E were intravenously injected with nitroglycerin 0.5μg/kg/min,1μg/kg/min and 2μg/min in 30 min respectively, and group A and B was injected with Sodium Chloride 0.5ml. The indexes of changed pathomorphology and ET/NO, TXB2/6-keto-PGF1a, were determined at the 6th and 12th hour after operations, respectively. Results The specimen data of the 6th and 12th hour displayed that the indexes of changed pathomorphology, ET, ET/NO, TXB2, and TXB2/6-keto-PGF1a of the group C,D and E degraded respectively, compared to group B(P < 0.05). Conclusion The nitroglycerin could degrade ET, ET/NO,TXA2 and TXA2/PGI2, improve the microcirculation of pancreas, and delay the pathological inflammation change in SAP rats.