丹酚酸B对内皮细胞氧化损伤的保护作用及机制研究

目的:研究丹酚酸B对人脐静脉内皮细胞(HUVECs)氧化损伤的保护作用,并探讨其对HUVECs CD54表达和中性粒细胞黏附率的影响.方法:以H2O2为损伤剂,观察丹酚酸B对HUVECs培养上清中LDH、NO、MDA含量的影响,并利用免疫组化方法和显微计数法观察该药对H2O2存在下CD54表达和中性粒细胞黏附率的影响.结果:丹酚酸B可剂量依赖性地减少H2O2所致内皮细胞LDH外漏和MDA生成,并提高NO释放,还能有效抑制H2O2存在下CD54表达和增加中性粒细胞黏附率.结论:丹酚酸B具有减轻H2O2所致内皮细胞的氧化性损伤,降低血管内皮细胞表面细胞黏附分子表达和抑制中性粒细胞黏附的作用,这一作用可能是丹酚酸B抗心肌缺血/再灌注损伤的作用机制之一.

Protective effect of salvianolic acid B on oxidative injury in HUVECs and its possible mechanism

ObjectivesTo study the effect of salvianolic acid B on oxidative injury induced by hydrogen peroxide and to investigate the HUVECs CD54 expression and the adherent percentage of neutrophil to HUVECs under oxidative stress.Methods: LDH leakage,NO release and MDA production in HUVECs supernatant were determined by different kits according to manufacture's introductions.HUVECs CD54 expression was measured by immunohistochemistry and neutrophil adherent percentage was calculated under microscope.Results:Salvianolic acid B could reduce the LDH leakage/MDA production and improve NO release in the presence of H2O2 in HUVECs.Additionally,it suppressed the HUVECs CD54 expression and the adherent percentage of neutrophil to HUVECs.Conclusion:Salvianolic acid B has a beneficial effect on oxidative injury induced by H2O2 in HUVECs,which may contribute to its effectiveness on ischemia-reperfusion damage.